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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cellulolytic enzyme production from agricultural residues for biofuel purpose on circular economy approach
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Cellulolytic enzyme production from agricultural residues for biofuel purpose on circular economy approach

机译：循环经济法从农业废渣生产纤维素酶用于生物燃料

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摘要

This study evaluated the production of cellulolytic enzymes from different agricultural residues. The crude enzyme extract produced was characterized and applied for saccharification of some agricultural residues. Maximum cellulolytic activities were obtained using soybean hulls. All enzymatic activities were highly stable at 40 degrees C at a pH range of 4.5-5.5. For stability at low temperatures, the enzyme extract was stored at freezing temperature and cooling for about 290 days without major loss of activity. The K-m values found for total cellulase (FPase), endoglucanase (CMCase), and xylanase were 19.73mgml(-1), 0.65mgml(-1), and 22.64mgml(-1), respectively, and V-max values were 0.82molmin(-1)mg(-1), 0.62molmin(-1)mg(-1), and 104.17molmin(-1)mg(-1) to cellulose, carboxymethyl cellulose, and xylan, respectively. In the saccharification tests, the total amount of total reducing sugars (TRS) released from 1g of soybean hulls catalyzed by the enzymes present in the crude enzyme extract was 0.16gg(-1) dry substrate.
机译：这项研究评估了不同农业残留物中纤维素分解酶的产生。对生产的粗酶提取物进行表征，并将其糖化用于一些农业残留物。使用大豆皮可获得最大的纤维素分解活性。在40摄氏度，pH范围为4.5-5.5时，所有酶活性均高度稳定。为了在低温下稳定，将酶提取物在冷冻温度下保存约290天，而活性没有重大损失。总纤维素酶（FPase），内切葡聚糖酶（CMCase）和木聚糖酶的Km值分别为19.73mgml（-1），0.65mgml（-1）和22.64mgml（-1），V-max值为0.82 molmin（-1）mg（-1），0.62molmin（-1）mg（-1）和104.17molmin（-1）mg（-1）分别对纤维素，羧甲基纤维素和木聚糖。在糖化试验中，由粗酶提取物中存在的酶催化的1克大豆壳释放的总还原糖（TRS）总量为0.16gg（-1）干燥底物。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2019年第5期|677-685|共9页
  • 作者

    Astolfi Viviane; Astolfi Angela Luiza; Mazutti Marcio A.; Rigo Elisandra; Di Luccio Marco; Camargo Aline Frumi; Dalastra Caroline; Kubeneck Simone; Fongaro Gislaine; Treichel Helen;

  • 作者单位

    Univ Reg Integrada Alto Uruguai & Missoes, Dept Food Engn, Campus Erechim,Av Sete Setembro 1621, BR-99700000 Erechim, RS, Brazil;

    Univ Reg Integrada Alto Uruguai & Missoes, Dept Food Engn, Campus Erechim,Av Sete Setembro 1621, BR-99700000 Erechim, RS, Brazil;

    Univ Fed Santa Maria, Dept Chem Engn, Av Roraima 1000, BR-97105900 Santa Maria, RS, Brazil;

    UDESC, Dept Food Engn, Linha Santa Terezinha, BR 282,Km 573-7, BR-89870000 Pinhalzinho, SC, Brazil;

    Univ Fed Santa Catarina, Dept Chem & Food Engn, BR-88800000 Florianopolis, SC, Brazil;

    Fed Univ Fronteira Sul, Lab Microbiol & Bioproc, ERS 135 Km 72,200, BR-99700000 Erechim, Brazil;

    Fed Univ Fronteira Sul, Lab Microbiol & Bioproc, ERS 135 Km 72,200, BR-99700000 Erechim, Brazil;

    Fed Univ Fronteira Sul, Lab Microbiol & Bioproc, ERS 135 Km 72,200, BR-99700000 Erechim, Brazil;

    Fed Univ Fronteira Sul, Lab Microbiol & Bioproc, ERS 135 Km 72,200, BR-99700000 Erechim, Brazil;

    Fed Univ Fronteira Sul, Lab Microbiol & Bioproc, ERS 135 Km 72,200, BR-99700000 Erechim, Brazil;

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  • 正文语种 eng
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  • 关键词

    Trichoderma reesei NRRL 3652; Solid-state fermentation; Saccharification; Agricultural residues;

    机译：里氏木霉NRRL 3652;固态发酵;糖化;农业残留物;

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