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首页> 外文期刊>Bioprocess and Biosystems Engineering >Role of media composition in biomass and astaxanthin production of Haematococcus pluvialis under two-stage cultivation
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Role of media composition in biomass and astaxanthin production of Haematococcus pluvialis under two-stage cultivation

机译：两阶段培养条件下培养基组成在雨生红球菌生物量和虾青素生产中的作用

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摘要

In the present study, the effects of four different culture media on the growth, astaxanthin production and morphology of Haematococcus pluvialis LUGU were studied under two-step cultivation. The interactions between astaxanthin synthesis and secondary messengers, reactive oxygen species (ROS) and mitogen-activated protein kinases (MAPK) were also investigated. In the first green vegetative cell stage, maximal biomass productivity (86.54mgL(-1)day(-1)) was obtained in BBM medium. In the induction stage, the highest astaxanthin content (21.5mgg(-1)) occurred in BG-11 medium, which was higher than in any other media. The expressions of MAPK and astaxanthin biosynthetic genes in BG-11 were higher than in any other media, whereas the ROS content was lower. Biochemical and physiological analyses suggested that the ROS, MAPK and astaxanthin biosynthetic gene expression was involved in astaxanthin biosynthesis in H. pluvialis under different culture media conditions. This study proposes a two-step cultivation strategy to efficiently produce astaxanthin using microalgae.
机译：在本研究中，通过两步培养研究了四种不同培养基对雨生红球菌LUGU的生长，虾青素产生和形态的影响。还研究了虾青素合成与次级信使，活性氧（ROS）和促分裂原活化蛋白激酶（MAPK）之间的相互作用。在第一个绿色营养细胞阶段，在BBM培养基中获得最大的生物量生产力（86.54mgL（-1）day（-1））。在诱导阶段，虾青素含量最高（21.5mgg（-1））出现在BG-11培养基中，高于其他任何培养基。 BG-11中MAPK和虾青素生物合成基因的表达高于其他任何培养基，而ROS含量较低。生化和生理分析表明，在不同培养基条件下，ROS，MAPK和虾青素的生物合成基因表达参与了幽门螺杆菌中虾青素的生物合成。这项研究提出了两步培养策略，以利用微藻高效生产虾青素。

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