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首页> 外文期刊>Bioprocess and Biosystems Engineering >Fermentation optimization and kinetic model for high cell density culture of a probiotic microorganism: Lactobacillus rhamnosus LS-8
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Fermentation optimization and kinetic model for high cell density culture of a probiotic microorganism: Lactobacillus rhamnosus LS-8

机译：益生菌微生物鼠李糖乳杆菌LS-8高细胞密度培养的发酵优化和动力学模型

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摘要

To develop a practical food-grade medium and optimal fermentation process for the cost-effective fermentation of Lactobacillus rhamnosus LS-8, both culture medium and conditions were optimized by combining single-factor experimental design, Plackett-Burman design and Box-Behnken design. The medium was simplified to five ingredients (g/L): whey powder (62.5), maltose syrup (50), corn steep liquor (55), NaCl (1) and lysine (0.05), and the optimal culture conditions were initial pH (6.28), constant fermentation pH (4.7), neutralizing agent (NaOH), aeration rate (0.2 v/v/min) and stirrer speed (200 rpm). After culturing in this optimized medium and conditions, the cell density of L. rhamnosus LS-8 was improved to 4.5 x 10(9) CFU/mL, which was elevated about 9 times higher than that obtained in MRS medium. Moreover, cell growth and substrate consumption kinetic constants were determined by the logistic equation and Luedeking-Piret model, and the R-2 values from the model equation were 0.9900 and 0.9971, respectively, indicating that these models were able to simulate the growth and substrate consumption of L. rhamnosus LS-8 accurately. In addition, a high-efficient production process of L. rhamnosus LS-8 was developed by repeated-batch operation, which was verified by five cycles of fermentation with good stability and repeatability. In conclusion, the efficiency of L. rhamnosus LS-8 fermentation was greatly improved as well as the reduction of the cost using the medium and process developed in the present study.
机译：为了开发一种经济实用的鼠李糖乳杆菌LS-8发酵的食品级培养基和最佳发酵工艺，通过结合单因素实验设计，Plackett-Burman设计和Box-Behnken设计来优化培养基和条件。将培养基简化为五种成分（g / L）：乳清粉（62.5），麦芽糖浆（50），玉米浆（55），NaCl（1）和赖氨酸（0.05），最佳培养条件为初始pH （6.28），恒定发酵pH（4.7），中和剂（NaOH），通气速率（0.2 v / v / min）和搅拌器速度（200 rpm）。在优化的培养基和条件下培养后，鼠李糖乳杆菌LS-8的细胞密度提高到4.5 x 10（9）CFU / mL，比MRS培养基高约9倍。此外，通过logistic方程和Luedeking-Piret模型确定细胞生长和基质消耗动力学常数，模型方程的R-2值分别为0.9900和0.9971，表明这些模型能够模拟生长和基质准确食用鼠李糖乳杆菌LS-8。此外，通过重复分批操作开发了鼠李糖乳杆菌LS-8的高效生产工艺，该工艺已通过五个发酵周期进行了验证，具有良好的稳定性和可重复性。总之，使用本研究开发的培养基和方法，鼠李糖乳杆菌LS-8发酵效率得到了极大的提高，并且降低了成本。

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