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首页> 外文期刊>Bioprocess and Biosystems Engineering >Characterization of microbial community dynamics during the anaerobic co-digestion of thermally pre-treated slaughterhouse wastes with glycerin addition
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Characterization of microbial community dynamics during the anaerobic co-digestion of thermally pre-treated slaughterhouse wastes with glycerin addition

机译：甘油加入热预处理的屠宰场废弃物的微生物群体动力学的表征

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摘要

Microbial community dynamics during the anaerobic co-digestion of pig manure, pasteurized slaughterhouse waste and glycerin were studied in a lab-scale CSTR. The feed composition was optimized through progressive co-substrate additions for enhanced methane production and organic matter removal without accumulation of intermediate compounds. Microbial community structure of biomass samples was studied by means of qPCR and DGGE profiling of 16S rRNA genes (Bacteria and Archaea), and genus-specific qPCR of the methyl coenzyme M reductase gene (mcrA), which encodes for an enzyme universally involved in methanogenesis. The composition of the dominant bacterial populations remained relatively stable, when compared to those in the influent, but the highest changes were observed upon the introduction of glycerin. Biodiversity of archaea was restricted to a few representatives of the genera Methanosaeta and Methanosarcina, but Methanospirillum sp. was detected only when glycerin was introduced in the feeding. Glycerin supplementation coincided with the strongest increase in methane yield (from 0.22 to 0.64m(CH4)(3)m(-3)d(-1)).
机译：在实验室规模CSTR中研究了猪粪，巴氏杀菌屠宰场废物和甘油的厌氧共同消化过程中的微生物群落动态。通过进行渐进的甲烷生产和有机物质去除而不会积聚中间体化合物，通过渐进式副衬底添加优化进料组合物。通过QPCR和DGGE谱分析为16S rRNA基因（细菌和古痤疮）的微生物群落结构，以及甲基辅酶M还原酶基因（MCRA）的特异性QPCR，其编码普遍参与甲烷的酶。与流入物的那些相比，显性细菌种群的组成仍然相对稳定，但在引入甘油时观察到最高的变化。 Archaea的生物多样性仅限于世代甲蛋白酶和甲蛋白酶的少数代表，但甲烷苏氏菌菌。仅在喂食中引入甘油时被检测到。甘油补充剂与甲烷产率的最强增加（0.22至0.64米（3）（3）m（3）℃（-1））。

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