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首页> 外文期刊>Bioprocess and Biosystems Engineering >Agricultural waste materials enhance protease production by Bacillus subtilis 622 in submerged fermentation under blue light-emitting diodes
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Agricultural waste materials enhance protease production by Bacillus subtilis 622 in submerged fermentation under blue light-emitting diodes

机译：农业废料在蓝色发光二极管下枯萎枯草芽孢杆菌622增强蛋白酶生产

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摘要

Bacillus bacteria have major utility in large-scale production of industrial enzymes, among which proteases have particular importance. B. subtilis B22, an aerobic and chemotrophic strain, was isolated from kimchi and identified by 16S rRNA gene sequencing. Extracellular protease production was determined in basic medium, with 1% (w/v) casein as substrate, by submerged fermentation at 37 degrees C under blue, green, red and white light-emitting diodes (LEDs), white fluorescent light and darkness. Fermentation under blue LEDs maximized protease production (110.79 +/- 1.8 U/mL at 24 h). Various agricultural waste products enhanced production and groundnut oil cake yielded the most protease (334 +/- 1.8 U/mL at 72 h). Activity and stability of the purified protease were optimum at pH 7-10 and 20-60 degrees C. Activity increased in the presence of Ca2+, Mg2+ and Mn2+, while Fe2+, Zn2+, Co2+ and Cu2+ moderated activity, and Ni2+ and Hg2+ inhibited activity. Activity was high (98%) in the presence of ethylenediaminetetraacetic acid (EDTA) but inhibited by phenylmethanesulfonyl fluoride (PMSF). The protease was unaffected by nonionic surfactants, tolerated an anionic surfactant and oxidizing agents, and was compatible with multiple organic solvents. These properties suggest utility of protease produced by B. subtilis B22 under blue LEDs for industrial applications.
机译：芽孢杆菌细菌在大规模生产的工业酶生产中具有重大效用，其中蛋白酶具有特别重要性。 B.枯草芽孢杆菌B22，一种有氧和嗜培菌株，从泡菜中分离，并鉴定为16S rRNA基因测序。在碱性培养基中测定细胞外蛋白酶生产，用1％（w / v）酪蛋白作为底物，在蓝色，绿色，红色和白色发光二极管（LED），白色荧光和暗度下的37℃下浸没。蓝色LED下的发酵最大化蛋白酶生产（24小时110.79 +/- 1.8 U / mL）。各种农业废品增强的生产和地生油饼产生最多的蛋白酶（72小时334 +/- 1.8 U / ml）。纯化蛋白酶的活性和稳定性在pH 7-10和20-60℃下最佳。在Ca2 +，Mg 2 +和Mn2 +的存在下，活性增加，而Fe 2 +，Zn2 +，CO 2 +和Cu2 +调节活性，以及​​Ni2 +和Hg2 +抑制活性。在存在乙二胺四乙酸（EDTA）的情况下，活性高（98％），但通过苯甲磺酰乙酰氟乙烯（PMSF）抑制。蛋白酶不受非离子表面活性剂的影响，可容忍阴离子表面活性剂和氧化剂，并与多种有机溶剂相容。这些特性建议使用B.枯草芽孢杆菌B22在蓝色LED下生产的蛋白酶用于工业应用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2020年第5期|821-830|共10页
  • 作者

    Elumalai Punniyakotti; Lim Jeong-Muk; Park Yool-Jin; Cho Min; Shea Patrick J.; Oh Byung-Taek;

  • 作者单位

    South China Normal Univ Sch Environm Guangzhou 510006 Guangdong Peoples R China|Chonbuk Natl Univ Adv Inst Environm & Biosci Div Biotechnol Coll Environm & Bioresource Sci Iksan 54596 Jeonbuk South Korea;

    Chonbuk Natl Univ Adv Inst Environm & Biosci Div Biotechnol Coll Environm & Bioresource Sci Iksan 54596 Jeonbuk South Korea;

    Chonbuk Natl Univ Dept Ecol Landscape Architecture Design Coll Environm & Bioresource Sci Iksan 54596 Jeonbuk South Korea;

    Chonbuk Natl Univ Adv Inst Environm & Biosci Div Biotechnol Coll Environm & Bioresource Sci Iksan 54596 Jeonbuk South Korea;

    Univ Nebraska Sch Nat Resources Lincoln NE 68583 USA;

    Chonbuk Natl Univ Adv Inst Environm & Biosci Div Biotechnol Coll Environm & Bioresource Sci Iksan 54596 Jeonbuk South Korea;

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  • 正文语种 eng
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  • 关键词

    Agricultural waste products; Blue LEDs; B; subtilis; Protease; Submerged fermentation;

    机译：农产品;蓝色LED;B;枯草芽孢杆菌;蛋白酶;浸没式发酵;

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