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首页> 外文期刊>Bioprocess and Biosystems Engineering >Transformation of ginsenoside via deep eutectic solvents based on choline chloride as an enzymatic reaction medium
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Transformation of ginsenoside via deep eutectic solvents based on choline chloride as an enzymatic reaction medium

机译：基于胆碱氯化胆碱的深共晶溶剂作为酶促反应介质的改变人参皂苷

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摘要

Ginsenoside compound K (CK) with a wide range of pharmacological activities has been widely used in the healthcare product industry. However, the application of CK is limited by low productivity and difficult separation. The purpose of this study is to convert ginsenoside Rb1 into CK by improving conversion efficiency in novel "green" reaction medium-deep eutectic solvent (DES). Talaromyces purpureogenus was selected from ginseng rhizosphere soil to produce beta-glucosidase with high activity and purity to transform ginsenosides, and Mn2+ was found to be an enzyme promoter. Among the DES based on choline chloride as hydrogen-bond receptor, choline chloride:ethylene glycol (ChCl:EG = 2:1) was the most promising solvent in maintaining enzyme activity and stability. In the presence of 30% v/v ChCl:EG = 2:1, the half-life of beta-glucosidase was increased by 96%, the solubility of F2 was increased by 120%, and CK yield was increased by 54% compared with those in the buffer. Fourier transform infrared, circular dichroism, and fluorescence spectroscopy confirmed that DES did not destroy the structure and conformation of beta-glucosidase. In addition, 80.6% CK conversion was obtained at 60 degrees C, pH 4.5, 48 h and 8 mM Rb1, which provided a feasible method for efficiently producing CK. Graphic abstract
机译：人参皂苷复合K（CK）具有广泛的药理学活性已广泛用于医疗保健产品行业。然而，CK的应用受到低生产率和困难分离的限制。本研究的目的是通过提高新型“绿色”反应介质 - 深对共晶溶剂（DES）中的转化效率将人参皂苷RB1转化为CK。 Talaromyces purpureogenus选自人参根际土壤，以生产β-葡糖苷酶，具有高活性和纯度来转化人参皂苷，并发现Mn2 +是酶启动子。在基于氯化胆碱作为氢粘合受体的DES中，氯化胆碱：乙二醇（CHCL：EG = 2：1）是保持酶活性和稳定性最有前途的溶剂。在30％V / V CCL的存在下：例如= 2：1，β-葡糖苷酶的半衰期增加了96％，F2的溶解度增加了120％，而CK产量比较54％与缓冲区中的那些。傅里叶变换红外，圆形二色性和荧光光谱证实，DES未破坏β-葡糖苷酶的结构和构象。另外，在60℃，pH 4.5,48 h和8mm RB1下获得80.6％CL转化，其提供了一种有效地生产CK的可行方法。图形摘要

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2020年第7期|1195-1208|共14页
  • 作者

    Ma Zhu; Mi Yu; Han Xin; Li Haohan; Tian Mi; Duan Zhiguang; Fan Daidi; Ma Pei;

  • 作者单位

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

    Northwest Univ Sch Chem Engn Shaanxi Key Lab Degradable Biomed Mat Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Shaanxi R&D Ctr Biomat & Fermentat Engn Sch Chem Engn Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China|Northwest Univ Biotech & Biomed Res Inst Taibai North Rd 229 Xian 710069 Shaanxi Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Talaromyces purpureogenus; Bioconversion; Ginsenoside CK; Deep eutectic solvent; beta-Glucosidase;

    机译：Talaromyces purpureogenus;生物转化;人参皂苷CK;深凝胶溶剂;β-葡糖苷酶;

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