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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced biochemical characteristics of β-glucosidase via adsorption and cross-linked enzyme aggregate for rapid cellobiose hydrolysis
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Enhanced biochemical characteristics of β-glucosidase via adsorption and cross-linked enzyme aggregate for rapid cellobiose hydrolysis

机译：通过吸附和交联酶聚集体增强β-葡糖苷酶的生化特征，用于快速纤维二糖水解

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摘要

With proper design, immobilization can be useful tool to improve the stability of enzymes, and in certain cases even their activity, selectivity, productivity and economic viability. An immobilized beta-glucosidase (BGL, EC 3.2.1.21) through matrix adsorption and cross-linked enzyme aggregate (ad-CLEA) technology is presented in this work. After adsorption and precipitation, BGL was immobilized to poly(glycidyl methacrylate-co-ethylenedimethacrylate) (P-GMA/EDMA) microparticles using glutaraldehyde as the cross-linker. Immobilized BGL exhibits lower apparentK(m)but much higherV(max)than that of the soluble enzyme, suggesting greater enzyme-substrate affinity and rapid velocity. Besides, ad-CLEA-BGL presents better thermostability retaining activity nearly 70% for 3 h and approximately 50% for 5 h at 70 degrees C, high operational reusability remaining more than 90% activity after nine uses and excellent storage stability holding about 95% activity after 45 days. Furthermore, the cellobiose is completely hydrolyzed within 1 h with ad-CLEA-BGL, which is significantly more efficient than soluble enzyme (about 4 h). Therefore, BGL was successfully immobilized onP(GMA/EDMA)microparticles with an ad-CLEA technology and the immobilization greatly enhances the biochemical characteristics. This work indicates promising application for ad-CLEA-BGL in utilizing agricultural remnants, bio-converting cellobiose to fermentable reducing sugar and ethanol production.[GRAPHICS].
机译：具有适当的设计，固定化可以是改善酶稳定性的有用工具，并且在某些情况下甚至其活动，选择性，生产力和经济可行性。通过基质吸附和交联酶聚集体（AD-CLEA）技术的固定化的β-葡糖苷酶（BGL，EC 3.2.1.21）。在吸附和沉淀后，使用戊二醛作为交联剂将BGL固定为聚（甲基丙烯酸缩水甘油酯 - 共乙基二甲基丙烯酸二甲基丙烯酸酯）（P-GMA / EDMA）微粒。固定化的BGL表现出低表达（M），但高于溶解酶的高（最大值），表明更大的酶底物亲和力和快速速度。此外，ad-clea-bgl呈现更好的热稳定性保持活性接近70％，持续3小时，约50％，在70摄氏度下，高于90％的活性高于90％，优异的储存稳定性保持约95％ 45天后的活动。此外，纤维纤维在1小时内完全水解，Ad-Clea-BgL，其比可溶性酶（约4小时）更有效。因此，BGL成功地固定了具有ad-CLEA技术的MICROMITIEND（GMA / EDMA）微粒，并且固定化大大提高了生物化学特性。这项工作表明，在利用农业残留，生物转化纤维糖和可发酵的还原糖和乙醇生产中，对AD-CLEA-BGL的承诺申请。[图形]。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2020年第12期|2209-2217|共9页
  • 作者

    Deng Xu; He Tian; Li Jun; Duan Hui-Ling; Zhang Zhi-Qi;

  • 作者单位

    Shaanxi Normal Univ Key Lab Analyt Chem Life Sci Shaanxi Prov Xian 710062 Peoples R China|Shaanxi Normal Univ Minist Educ Key Lab Med Resources & Nat Pharmaceut Chem Xian 710062 Peoples R China;

    Shaanxi Normal Univ Key Lab Analyt Chem Life Sci Shaanxi Prov Xian 710062 Peoples R China|Shaanxi Normal Univ Minist Educ Key Lab Med Resources & Nat Pharmaceut Chem Xian 710062 Peoples R China;

    Shaanxi Normal Univ Key Lab Analyt Chem Life Sci Shaanxi Prov Xian 710062 Peoples R China|Shaanxi Normal Univ Minist Educ Key Lab Med Resources & Nat Pharmaceut Chem Xian 710062 Peoples R China;

    Shaanxi Normal Univ Key Lab Analyt Chem Life Sci Shaanxi Prov Xian 710062 Peoples R China|Shaanxi Normal Univ Minist Educ Key Lab Med Resources & Nat Pharmaceut Chem Xian 710062 Peoples R China;

    Shaanxi Normal Univ Key Lab Analyt Chem Life Sci Shaanxi Prov Xian 710062 Peoples R China|Shaanxi Normal Univ Minist Educ Key Lab Med Resources & Nat Pharmaceut Chem Xian 710062 Peoples R China;

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  • 正文语种 eng
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  • 关键词

    beta-glucosidase; Adsorption; Cross-linked enzyme aggregates; Poly(glycidyl methacrylate-co-ethylenedimethacrylate) microparticles; Cellobiose; Ethanol production;

    机译：β-葡萄糖苷酶;吸附;交联酶聚集体;聚（甲基丙烯酸缩水甘油酯 - 共乙二二甲基丙烯酸酯）微粒;纤维素;乙醇生产;

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