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首页> 外文会议>European biomass conference and exhibition;ETA-Florence Renewable Energies >HYDROLYSIS OF CORN STOVER WITH ACREMONIUM CELLULASES: ANALYSIS OF THE RESIDUAL ENZYMES ACTIVITIES THROUGH THE PROCESS AND EFFECT OF ADDITIVES TO REDUCE THE ENZYMES UNSPECIFIC ADSORPTION
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HYDROLYSIS OF CORN STOVER WITH ACREMONIUM CELLULASES: ANALYSIS OF THE RESIDUAL ENZYMES ACTIVITIES THROUGH THE PROCESS AND EFFECT OF ADDITIVES TO REDUCE THE ENZYMES UNSPECIFIC ADSORPTION

机译：RE曲霉纤维素酶水解玉米秸秆：通过减少有害酶吸附过程和添加剂的作用分析残留酶的活性

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Sugars from biomass represent a versatile platform for the production of a number of bio-basedrnproducts. Cellulases enzymes play an important role as they can hydrolyse biomass at mild conditions. Cellulases arernmostly produced from Trichoderma and Aspergillus fungi. Although less investigated, another fungus, Acremoniumrncellulolyticus produces effective cellulases for the conversion of lignocellullosic substrates. The present paperrndescribes the conversion of corn stover steam pretreated by Acremonium enzymes. The performaces of thernAcremonium cellulases were compared with commercial Trichoderma and Aspergillus blends (Celluclast andrnNovozyme). The results indicated that, at the same process conditions, Acremonium cellulases were more effectivernand yielded 20% more sugars than the reference blends. Furthermore, the addition of external hemicellulasesrn(Multifect xylanase) to the Acremonium complex produced a xylan hydrolysis 20% higher than that produced by thernaddition of the same amount to the reference mixtures. These findings indicated an higher synergistic action of thernenzymatic components in the Acremonium complex with the commercial hemicellulases mixtures. In order to prolongrnthe enzymes life time during the enzymatic hydrolysis with the Acremonium complex, three additives, namely Tweenrn80, Tween 20 and BSA, were tested to reduce the unspecific enzymes adsorption. The residual enzymatic activities inrnthe hydrolysates were monitored during the hydrolysis and the results compared with control systems. The findingsrnindicated that the use of Tween 80 and Tween 20 were effective in reducing the adsorption of the cellobiosernhydrolases.
机译：来自生物质的糖代表了生产多种生物基产品的多功能平台。纤维素酶起重要作用，因为它们可以在温和的条件下水解生物质。纤维素酶主要来自木霉属和曲霉属真菌。尽管研究较少，但另一种真菌即解霉菌也能产生有效的纤维素酶，以转化木质纤维素底物。本文描述了用顶基酶预处理的玉米秸秆蒸汽的转化。将顶头纤维素酶的性能与商品木霉和曲霉混合物（Celluclast和rnNovozyme）进行了比较。结果表明，在相同的工艺条件下，顶孢纤维素酶比参考混合物更有效，糖产量比参考混合物高20％。此外，将外部半纤维素酶（Multifect木聚糖酶）添加到顶孢菌属复合物中产生的木聚糖水解比通过将相同量的参比混合物添加到参考混合物中产生的木聚糖水解高20％。这些发现表明，顶孢菌属复合物中的酶成分与商业半纤维素酶混合物具有更高的协同作用。为了延长酶在Acremonium复合物进行酶水解过程中的寿命，测试了三种添加剂Tweenrn80，Tween 20和BSA，以减少非特异性酶的吸附。在水解过程中监测水解产物中残留的酶活性，并将结果与​​对照系统进行比较。研究结果表明，使用吐温80和吐温20可有效减少纤维二糖水解酶的吸附。

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