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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Hydrolysis of Corn Stover by Talaromyces cellulolyticus Enzymes: Evaluation of the Residual Enzymes Activities Through the Process
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Hydrolysis of Corn Stover by Talaromyces cellulolyticus Enzymes: Evaluation of the Residual Enzymes Activities Through the Process

机译：塔罗米斯纤维素溶解酶的玉米秸秆水解：通过该过程评价残留酶活性

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摘要

The obtainment of sugars from lignocellulosic residues represents a sustainable and versatile platform for the production of a number of bio-based products. Cellulases are a family of enzymes which can effectively hydrolyze the biomass polysaccharides at mild conditions. Cellulolytic fungi belonging to the genera Trichoderma and Aspergillus are the most commonly source of commercial cellulases used so far. More recently, Talaromyces cellulolyticus was also scored as a promising cellulases producer. In comparison to the Trichoderma and Aspergillus systems, Talaromyces enzymes have been less investigated. The present research dealt with the conversion of steam-pretreated corn stover by commercial blend of T. cellulolyticus enzymes with respect to the common blends. The paper also investigated the stability of the enzyme preparation and tested the use of additives (namely Tween 80, Tween 20, and BSA) to improve the enzymes performances and the hydrolysis efficiency. The results indicated that, at the same process conditions, T. cellulolyticus cellulases were more effective and yielded 20% more sugars compared to control blends. Furthermore, the cellulase components displayed a synergistic interaction with hemicellulases. The results indicate that cellulases from T. cellulolyticus are less affected by the high dry matter consistency and the use of additives could increase the total activity by around 50% and -glucosidase capacity by 10-15%.
机译：从木质纤维素残留物中获得糖的糖代表可持续和通用的平台，用于生产许多生物基产品。纤维素酶是一种酶系列，可以在温和条件下有效地水解生物质多糖。属于Genera Trichoderma和Aspergillus的纤维素溶解真菌是迄今为止使用的商业纤维素酶最常见的来源。最近，Talaromyces Cellulolyticus也被评为有前途的纤维素酶生产商。与Trichoderma和Aspergillus系统相比，缩略口酶已经不太研究。本研究涉及通过相对于常见共混物的T.Cellulolyticus酶的商业混合物转化蒸汽预处理玉米秸秆的转化。本文还研究了酶制剂的稳定性，并测试了添加剂（即吐温80，Tween 20和BSA）的使用，以改善酶的性能和水解效率。结果表明，在相同的过程条件下，与对照共混物相比，T. Cellulolyticus纤维素酶更有效并产生糖增加20％。此外，纤维素酶组分显示出与半纤维素酶的协同相互作用。结果表明，来自T.Cellulyticus的纤维素酶受到高干物质稠度的影响，并且添加剂的使用可以将总活性增加约50％和 - 葡糖苷酶容量10-15％。

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