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首页> 外文期刊>Bioprocess and Biosystems Engineering >Limited oxygen conditions as an approach to scale-up and improve d and L-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli
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Limited oxygen conditions as an approach to scale-up and improve d and L-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli

机译：有限的氧气条件作为扩大和改善矿物质培养基和牛油果种子水解产物的D和L-乳酸产生的方法与代谢工程的大肠杆菌

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摘要

The effectiveness of micro-aeration on lactate (LA) production by metabolically engineeredEscherichia coliwas evaluated in 1 L bioreactors containing mineral media and glucose (70 g/L). Volumetric oxygen transfer coefficients (k(L)a) between 12.6 and 28.7 h(-1)increased the specific growth rate (mu) and volumetric productivity (Q(LA)) by 300 and 400%, respectively, without a significant decrease in lactate yield (Y-LA), when compared with non-aerated fermentations. A k(L)a of 12.6 h(-1)was successfully used as a criterion to scale-up the production oflandd-lactate from 1 to 11 and 130 L. Approximately constant Q(LA)and Y(LA)values were obtained throughout the fermentation scale-up process. Furthermore, ad-lactogenic fermentation was carried out in 1 L bioreactors using avocado seed hydrolysate as a culture medium under the same k(L)a value, displaying high Q(LA)and Y-LA.
机译：微通气在含有矿物质培养基和葡萄糖（70g / L）的1L生物反应器中评估了代谢工程晶体Coliwas对乳酸（La）产生的有效性。在12.6和28.7h（-1）之间的体积氧转移系数（K（l）a）（-1）增加了比较的生长速率（mu）和体积生产率（q（la）），分别为300％和400％，没有显着下降与非充气发酵相比，乳酸产率（Y-LA）。成功使用12.6小时（-1）的K（L）A为标准，从1至11和130L扩大到乳酸盐的生产。获得近似恒定的Q（La）和Y（La）值在整个发酵放大过程中。此外，使用鳄梨种子水解产物作为培养基在同一K（l）的培养基中，显示高Q（la）和y-la的培养基，在1μl生物反应器中进行酰肠粘发酵。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2021年第2期|379-389|共11页
  • 作者

    Sierra-Ibarra Estefania; Leal-Reyes Laura J.; Huerta-Beristain Gerardo; Hernandez-Orihuela Ana L.; Gosset Guillermo; Martinez-Antonio Agustino; Martinez Alfredo;

  • 作者单位

    Univ Nacl Autonoma Mexico Dept Ingn Celular & Biocatalisis Inst Biotecnol Ave Univ 2001 Cuernavaca 62210 Morelos Mexico;

    Univ Nacl Autonoma Mexico Dept Ingn Celular & Biocatalisis Inst Biotecnol Ave Univ 2001 Cuernavaca 62210 Morelos Mexico;

    Univ Nacl Autonoma Mexico Dept Ingn Celular & Biocatalisis Inst Biotecnol Ave Univ 2001 Cuernavaca 62210 Morelos Mexico|Univ Autonoma Guerrero Fac Ciencias Quim Biol Ave Lazaro Cardenas S-N Cd Univ Chilpancingo 39070 Guerrero Mexico;

    Inst Politecn Nacl Dept Ingn Genet Ctr Invest & Estudios Avanzados Unidad Irapuato Km 9-6 Libramiento Norte Carretera Irapuato Leon Guanajuato 36821 Mexico;

    Univ Nacl Autonoma Mexico Dept Ingn Celular & Biocatalisis Inst Biotecnol Ave Univ 2001 Cuernavaca 62210 Morelos Mexico;

    Inst Politecn Nacl Dept Ingn Genet Ctr Invest & Estudios Avanzados Unidad Irapuato Km 9-6 Libramiento Norte Carretera Irapuato Leon Guanajuato 36821 Mexico;

    Univ Nacl Autonoma Mexico Dept Ingn Celular & Biocatalisis Inst Biotecnol Ave Univ 2001 Cuernavaca 62210 Morelos Mexico;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Escherichia coli; Lactic acid; Scale-up; Avocado seed hydrolysate; Volumetric oxygen transfer coefficient;

    机译：大肠杆菌;乳酸;鳞片;鳄梨种子水解产物;体积氧转印系数;

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