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A low-cost brewery waste as a carbon source in bio-surfactant production

机译:低成本的啤酒厂废物作为生物表面活性剂生产中的碳源

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This work aims to produce bio-surfactant using a brewery waste (trub) as a strategy to reduce production costs related to the substrate, as well as to provide an eco-friendly destination for this residue. Trub is obtained during the boiling of the wort, being mainly composed of proteins and reducing sugars. To evaluate important process parameters on bio-surfactant production, a full factorial design (2(4)) was elaborated, having agitation rate and concentrations of trub, yeast extract, and peptone as independent variables. The highest bio-surfactant concentration achieved was 100.76 mg L-1, where FTIR and Maldi-ToF-MS confirmed functional groups characteristic of peptides and isomers of surfactin in the bio-surfactant extract. Trub, agitation and yeast extract showed statistically significant effects on the response variable (surface tension), where an increase in the agitation rate and in the concentration of yeast extract demonstrated a positive impact on the production of bio-surfactant.
机译:这项工作旨在使用啤酒厂废物(TRUB)制造生物表面活性剂作为降低与基材相关的生产成本的策略,以及为该残留物提供环保型目的地。在麦芽汁的沸腾过程中获得Trub,主要由蛋白质和还原糖组成。为了评估生物表面活性剂生产的重要过程参数,详细阐述了完整的因子(2(4)),具有搅拌速率和Trub,酵母提取物和蛋白胨作为独立变量的浓度。获得的最高生物表面活性剂浓度为100.76mg L-1,其中FTIR和MALDI-TOF-MS确认了生物表面活性剂提取物中的肽和SUSFACTIN的异构体的官能团。 TRUB,搅拌和酵母提取物对响应变量(表面张力)显示出统计学上显着的影响,其中搅拌速率和酵母提取物浓度的增加表现出对生物表面活性剂的产生的积极影响。

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