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首页> 外文期刊>Biotechnology and bioprocess engineering >Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures
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Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures

机译:分批和补料分批培养法通过工程化全甲羟戊酸途径的重组大肠杆菌生产β-胡萝卜素

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摘要

Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for p-carotene biosynthesis, was utilized for production of p-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37℃ were 1.7-fold and 2-fold higher, respectively, than those at 28℃. Glycerol was superior to glucose as a carbon source. Maximum p-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.
机译:经过工程改造以包含完整的甲羟戊酸途径和用于p-胡萝卜素生物合成的外源基因的重组大肠杆菌被用于在生物反应器培养物中生产p-胡萝卜素。在分批和pH固定进料分批培养中确定了最佳培养条件,以确定最佳的进料策略,从而提高了产量。在37℃下,分批培养的比生长速率和体积生产率分别比28℃下高1.7倍和2倍。甘油作为碳源优于葡萄糖。通过在pH恒定的分批培养中同时添加500 g / L甘油和50 g / L酵母提取物,可实现最大的对胡萝卜素产量(滴度663 mg / L,总体积生产率24.6 mg / L×h) 。

著录项

  • 来源
    《Biotechnology and bioprocess engineering》 |2009年第5期|559-564|共6页
  • 作者单位

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

    Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea;

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

    Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 660-701, Korea;

    Department of Electronic and Communications Engineering, Kwangwoon University, Seoul 139-701, Korea;

    School of Electrical Engineering, Kookmin University, Seoul 136-702, Korea;

    Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    recombinant escherichia coli; engineered whole mevalonate pathway; p-carotene; bioreactor culture; fed-batch culture;

    机译:重组大肠杆菌设计的全甲羟戊酸途径;对胡萝卜素;生物反应器培养;分批补料培养;

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