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首页> 外文期刊>British Journal of Pharmacology >Calcium-pH crosstalks in rat mast cells: cytosolic alkalinization, but not intracellular calcium release, is a sufficient signal for degranulation.
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Calcium-pH crosstalks in rat mast cells: cytosolic alkalinization, but not intracellular calcium release, is a sufficient signal for degranulation.

机译：大鼠肥大细胞中的钙pH值串扰：胞质碱化而不是细胞内钙释放是脱颗粒的充分信号。

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摘要

The aim of this work was to study the relationship between intracellular alkalinization, calcium fluxes and histamine release in rat mast cells. Intracellular alkalinization was induced by nigericin, a monovalent cation ionophore, and by NH(4)Cl (ammonium chloride). Calcium cytosolic and intracellular pH were measured by fluorescence digital imaging using Fura-2-AM and BCECF-AM. In rat mast cells, nigericin and NH(4)Cl induce a dose-dependent intracellular alkalinization, a dose-dependent increase in intracellular calcium levels by releasing calcium from intracellular pools, and an activation of capacitative calcium influx. The increase in both intracellular calcium and pH activates exocytosis (histamine release) in the absence of external calcium. Under the same conditions, thapsigargin does not activate exocytosis, the main difference being that thapsigargin does not alkalinize the cytosol. After alkalinization, histamine release is intracellular-calcium dependent. With 2.5 mM EGTA and thapsigargin the cell response decreases by 62%. The cytosolic alkalinization, in addition to the calcium increase it is enough signal to elicit the exocytotic process in rat mast cells.
机译：这项工作的目的是研究大鼠肥大细胞中细胞内碱化，钙通量和组胺释放之间的关系。细胞内碱化是由尼日尔菌素，一价阳离子离子载体和NH（4）Cl（氯化铵）诱导的。使用Fura-2-AM和BCECF-AM通过荧光数字成像测量钙质和细胞内pH。在大鼠肥大细胞中，尼日利亚霉素和NH（4）Cl诱导剂量依赖性细胞内碱化，通过从细胞内池中释放钙来剂量依赖性地增加细胞内钙水平以及激活钙离子内流。在没有外部钙的情况下，细胞内钙和pH的增加会激活胞吐作用（组胺释放）。在相同条件下，thapsigargin不会激活胞吐作用，主要区别在于thapsigargin不会碱化细胞溶质。碱化后，组胺释放是细胞内钙依赖性的。使用2.5 mM EGTA和毒胡萝卜素，细胞反应降低62％。除钙增加外，胞浆碱化还足以引发大鼠肥大细胞的胞吐过程。

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