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Sonoporation at a low mechanical index

机译：机械指数低时的声纳

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摘要

The purpose of this study was to investigate the physical mechanisms of sonoporation, in order to understand and improve ultrasound-assisted drug and gene delivery. Sonoporation is the transient permeabilisation and resealing of a cell membrane with the help of ultrasound and/or an ultrasound contrast agent, allowing for the trans-membrane delivery and cellular uptake of macromolecules between 10 kDa and 3 MDa. The authors studied the behaviour of ultrasound contrast agent microbubbles near cancer cells at low acoustic amplitudes. After administering an ultrasound contrast agent, HeLa cells were subjected to 6-6 MHz ultrasound with a mechanical index of 0-2 and observed with a high-speed camera. Microbubbles were seen to enter cells and rapidly dissolve. The quick dissolution after entering suggests that the microbubbles lose (part of) their shell while entering. The authors have demonstrated that lipid-shelled microbubbles can be forced to enter cells at a low mechanical index. Hence, if a therapeutic agent is added to the shell of the bubble or inside the bubble, ultrasound-guided delivery could be facilitated at diagnostic settings. In addition, these results may have implications for the safety regulations on the use of ultrasound contrast agents for diagnostic imaging.
机译：这项研究的目的是调查声穿孔的物理机制，以了解和改善超声辅助药物和基因的传递。超声操作是借助于超声和/或超声造影剂的瞬时透化和细胞膜的再密封，从而允许跨膜递送和10 kDa至3 MDa之间的大分子的细胞摄取。作者研究了超声造影剂微泡在癌细胞附近以低声振幅的行为。给予超声造影剂后，对HeLa细胞进行6-6 MHz超声，机械指数为0-2，并用高速相机观察。观察到微泡进入细胞并迅速溶解。进入后迅速溶解表明，微泡在进入时会失去壳的一部分。作者已经证明，带脂质的微泡可以被迫以低机械指数进入细胞。因此，如果将治疗剂添加到气泡的外壳或气泡的内部，则可以在诊断设置中促进超声引导的递送。此外，这些结果可能会影响超声造影剂用于诊断成像的安全规定。

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  • 来源
    《Bubble science, engineering & technology》 |2011年第1期|p.3-11|共9页
  • 作者

    A. Delalande; S. Kotopoulis; T. Rovers; C. Pichon; M. Postema;

  • 作者单位

    Centre de Biophysique Moleculaire, UPR 4301 CNRS affiliated to theUniversity of Orleans, rue Charles Sadron, 45071 Orleans Cedex 2,France;

    Department of Engineering, The University of Hull, Cottingham Road, Kingston upon Hull HU6 7RX, UK;

    Emmy Noether Research Group, Institute of Medical Engineering, Department' of Electrical Engineering and Information Sciences, Ruhr-Universitat Bochum, ID 04/24, 44780 Bochum, Germany;

    Centre de Biophysique Moleculaire, UPR 4301 CNRS affiliated to theUniversity of Orleans, rue Charles Sadron, 45071 Orleans Cedex 2,France;

    Centre de Biophysique Moleculaire, UPR 4301 CNRS affiliated to theUniversity of Orleans, rue Charles Sadron, 45071 Orleans Cedex 2,France,Department of Engineering, The University of Hull, Cottingham Road, Kingston upon Hull HU6 7RX, UK,Emmy Noether Research Group, Institute of Medical Engineering, Department' of Electrical Engineering and Information Sciences, Ruhr-Universitat Bochum, ID 04/24, 44780 Bochum, Germany,Department of Physics and Technology, University of Bergen, Allegaten55, 5007 Bergen, Norway;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    sonoporation; low mechanical index; microbubbles; ultrasound contrast agent; hela cells; cell penetration;

    机译：声纳机械指数低;微泡超声造影剂Hela细胞细胞渗透;

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