...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Advances: An International Review Journal >Ultrasound: mechanical gene transfer into plant cells by sonoporation.
【24h】

Ultrasound: mechanical gene transfer into plant cells by sonoporation.

机译：超声：通过声波穿透将机械基因转移到植物细胞中。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Development of nonviral gene transfer methods would be a valuable alternative of gene therapy or transformation. Ultrasound can produce a variety of nonthermal bioeffects via acoustic cavitation. Cavitation bubbles can induce cell death or transient membrane permeabilization (sonoporation) on cells. Application of sonoporation for gene transfer into cells or tissues develops quickly in recent years. Many studies have been performed in vitro exposure systems to a variety of cell lines transfected successfully. In vivo, cavitation initiation and control are more difficult, but can be enhanced by ultrasound contrast agents (microbubbles). The use of ultrasound for nonviral gene delivery has been applied for mammalian systems, which provides a fundamental basis and strong promise for development of new gene therapy methods for clinical medicine. In this paper, ultrasound applied to plant cell transformation or gene transfer is reviewed. Recently, most researches are focused on sonication-assisted Agrobacterium-mediated transformation (SAAT) in plant cells or tissues. Microbubbles are also proposed to apply to gene transfer in plant cells and tissues.
机译：非病毒基因转移方法的发展将是基因治疗或转化的有价值的替代方法。超声可通过声空化产生多种非热生物效应。空化气泡可以诱导细胞死亡或细胞上的瞬时膜通透性（sonoporation）。近年来，声波穿孔技术可用于将基因转移到细胞或组织中。在体外暴露于成功转染的多种细胞系的系统中已经进行了许多研究。在体内，空化的引发和控制较为困难，但可以通过超声造影剂（微泡）增强。超声用于非病毒基因递送已被应用于哺乳动物系统，这为开发临床医学新的基因治疗方法提供了基础和强大的前景。本文综述了超声在植物细胞转化或基因转移中的应用。近来，大多数研究集中在植物细胞或组织中的超声辅助农杆菌介导的转化（SAAT）。还提出将微泡应用于植物细胞和组织中的基因转移。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号