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首页> 外文期刊>World Journal of Gastroenterology >Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China
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Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China

机译：利用寡核苷酸芯片检测华东地区乙型肝炎病毒携带者的乙型肝炎病毒基因型

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AIM: To determine the genotype distribution of hepatitis B virus (HBV) with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS: An assay using oligonucleotide chip was developed for detection of HBV genotypes in serum samples from HBV DNA-positive patients in Eastern China. This method is based on the principle of reverse hybridization with Cy5-labeled amplicons hybridizing to type-specific oligonucleotide probes that are immobilized on slides. The results of 80 randomly chosen sera were confirmed by direct sequencing. RESULTS: HBV genotype B, C and mixed genotype were detected in 400 serum samples, accounting for 8.3% (n = 33), 83.2% (n = 333), and 8.5% (n = 34), respectively. The evaluation of the oligonucleotide assay showed 100% concordance with the amplicon phylogenetic analysis except 9 mixed genotype infections undetected by sequencing. CONCLUSION: The study indicates that HBV genotype C and B prevail in the Eastern China. It is suggested that the oligonucleotide chip is a reliable and convenient tool for the detection of HBV genotyping.
机译：目的：通过一种新的寡核苷酸芯片检测方法确定华东地区乙型肝炎病毒携带者中乙型肝炎病毒（HBV）的基因型分布。方法：开发了一种使用寡核苷酸芯片的检测方法，用于检测华东地区HBV DNA阳性患者血清样本中的HBV基因型。该方法基于与Cy5标记的扩增子反向杂交的原理，该扩增子与固定在载玻片上的类型特异性寡核苷酸探针杂交。通过直接测序确认了80个随机选择的血清的结果。结果：在400份血清样本中检测到HBV B，C和混合基因型，分别占8.3％（n = 33），83.2％（n = 333）和8.5％（n = 34）。寡核苷酸测定的评估显示与扩增子系统发育分析的一致性为100％，除了9种混合基因型感染未通过测序发现。结论：研究表明，乙型肝炎病毒基因型C和B在华东地区盛行。提示寡核苷酸芯片是检测HBV基因型的可靠且方便的工具。

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