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首页> 外文期刊>World Journal of Gastroenterology >Clinical significance of telomerase activity in peritoneal lavage fluid from patients with gastric cancer and its relationship with cellular proliferation
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Clinical significance of telomerase activity in peritoneal lavage fluid from patients with gastric cancer and its relationship with cellular proliferation

机译：胃癌患者腹腔灌洗液中端粒酶活性的临床意义及其与细胞增殖的关系

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AIM: To evaluate the efficacy of telomerase activity assay and peritoneal lavage cytology (PLC) examination in peritoneal lavage fluid for the prediction of peritoneal metastasis in gastric cancer patients, and to explore the relationship between telomerase activity and proliferating cell nuclear antigen expression. METHODS: Telomeric repeated amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA) was performed to measure the telomerase activity in 60 patients with gastric cancer and 50 with peptic ulcer. PLC analysis of the 60 patients with gastric cancer was used for comparison. The proliferating cell nuclear antigen (PCNA) in gastric carcinoma was immunohistochemically examined. RESULTS: The telomerase activity and PLC positive rate in peritoneal lavage fluid from patients with gastric cancer was 41.7% (25/60), and 25.0% (15/60), respectively. The positive rate of telomerase activity was significantly higher than that of PLC in the group of pT_4 (15/16 vs 9/16, P < 0.05), P_(1-3) (13/13 vs 9/13, P < 0.05) and diffuse type (22/42 vs 13/42, P < 0.05). The patients with positive telomerase activity, peritoneal metastasis, and serosal invasion had significantly higher levels of average PCNA proliferation index (PI), (55.00 ± 6.59 vs 27.43 ± 7.72, 57.26 ± 10.18 vs 29.15 ± 8.31, and 49.82 ± 6.74 vs 24:65 ± 7.33, respectively, P < 0.05). CONCLUSION: The TRAP assay for telomerase activity is a useful adjunct for cytologic method in the diagnosis of peritoneal micrometastasis and well related to higher proliferating activity of gastric cancer. The results of this study also suggest a promising future therapeutic strategy for treating peritoneal dissemination based on telomerase inhibition.
机译：目的：评估端粒酶活性测定和腹腔灌洗液中的腹腔灌洗细胞学（PLC）检查对胃癌患者腹膜转移的预测效果，并探讨端粒酶活性与增殖细胞核抗原表达之间的关系。方法：采用端粒重复扩增法（TRAP）-酶联免疫吸附法（ELISA）检测60例胃癌和50例消化性溃疡患者的端粒酶活性。比较60例胃癌患者的PLC分析。免疫组化检查了胃癌中增殖细胞核抗原（PCNA）。结果：胃癌患者腹腔灌洗液中端粒酶活性和PLC阳性率分别为41.7％（25/60）和25.0％（15/60）。 pT_4（15/16 vs 9/16，P <0.05），P_（1-3）（13/13 vs 9/13，P <0.05）组的端粒酶活性阳性率显着高于PLC ）和漫射类型（22/42与13/42，P <0.05）。端粒酶活性，腹膜转移和浆膜浸润阳性的患者的平均PCNA增殖指数（PI）显着较高（（55.00±6.59 vs 27.43±7.72、57.26±10.18 vs 29.15±8.31和49.82±6.74 vs 24：分别为65±7.33，P <0.05）。结论：TRAP检测端粒酶活性是细胞学方法在腹膜微转移诊断中的有用辅助手段，与胃癌的高增殖活性密切相关。这项研究的结果还表明，基于端粒酶抑制作用的腹膜扩散治疗有前途的治疗策略。

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