...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Establishing models of portal vein occlusion and evaluating value of multi-slice CT in hepatic VX2 tumor in rabbits
【24h】

Establishing models of portal vein occlusion and evaluating value of multi-slice CT in hepatic VX2 tumor in rabbits

机译：家兔肝VX2肿瘤门静脉阻塞模型的建立及多层CT的评价价值

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To establish models of portal vein occlusion of hepatic VX2 tumor in rabbits and to evaluate the value of multi-slice CT. METHODS: Forty New Zealand rabbits were divided into 4 groups according to digital table: Immediate group (group A; transplantation of tumor immediately after the portal vein occlusion), 3-wk group (group B; transplantation of tumor at 3 wk after the portal vein occlusion), negative control group (group C) and positive control group (group D), 10 rabbits in each group. Hepatic VX2 tumor was transplanted with abdominal-embedding innoculation immediately after the portal vein occlusion and at 3 wk after the portal vein occlusion. Meanwhile, they were divided into negative control group (Left external branch of portal vein was occluded by sham-operation, and left exite was embedded and inoculated pseudoly) and positive control group (Transplanted tumor did not suffer from the portal vein occlusion). All rabbits were scanned with multi-slice CT. RESULTS: All 40 animals were employed in the final analysis without death. Tumor did not grow in both immediate group and 3-wk group. In 3-wk group, left endite was atrophied and growth of tumor was inhibited. The maximal diameter of tumor was significantly smaller than that in positive control group (2.55 ± 0.46 vs 3.59 ± 0.37 cm, t = 5.57, P < 0.001). Incidences of metastasis in the liver and lung were lower in 3-wk group than those in positive control group (10% vs 40%, and 90% vs 100%, respectively). The expression intensities of the vascular endpthelium growth factor (VEGF) in groups A, B, C and D were 0.10 ± 0.06, 0.66 ± 0.21, 0.28 ± 0.09 and 1.48 ± 0.32, respectively. VEGF expression level in the test group A was significantly lower than that in the negative control group C (t = 5.07; P < 0.001). In addition, VEGF expression in the test group B was significantly lower than that in the positive control group D (t = 6.38; P < 0.001). Scanning with multi-slice CT showed that displaying rate of hepatic artery branches was obviously lower in grade Ⅲ (40%) than that in grade Ⅰ (70%) and Ⅱ (100%) (P < 0.05); but there was no significant difference in displaying rate of the portal vein at various grades. Values of blood flow (BF) of the liver, blood volume (BV), mean transit time (MTT) and permeability of vascular surface (PS) were lower in the immediate group and 3-wk group than those in control groups, but values of hepatic arterial fraction (HAF) were increased. Significant positive correlations were existed between BF and BV (r = 0.905, P < 0.01), and between BF and PS (r = 0.967, P < 0.01), between BV and PS (r = 0.889, P < 0.01). A significant negative correlation existed between PV and HAF (r = -0.768, P < 0.01), between PS and HAF (r = -0.557, P < 0.01). The values of BF, BV and PS had a positive correlation with VEGF (r_(BF) = 0.842, r_(BV) = 0.579, r_(PS) = 0.811, P < 0.01) . However, there was no significant correlation between the values of MTT and HAF and the VEGF expression (r_(MTT) = 0.066, r_(HAF) -0.027). CONCLUSION: Ligating the left external branch of portal vein is an ideal way to establish models of portal vein occlusion in rabbits with hepatic VX2 tumor. Multi-slice CT plays a key role in evaluating effect of portal vein occlusion.
机译：目的：建立家兔肝VX2肿瘤门静脉阻塞模型，评价多层CT的价值。方法：40只新西兰大白兔根据数字表分为4组：即刻组（A组；门静脉闭塞后立即移植肿瘤）； 3周组（B组；门脉后3周移植瘤）。静脉阻塞），阴性对照组（C组）和阳性对照组（D组），每组10只。在门静脉闭塞后立即和门静脉闭塞后第3周，将肝VX2肿瘤移植入腹部嵌入接种物中。同时，将其分为阴性对照组（假手术阻塞门静脉左外分支，假出口并假接种左出口）和阳性对照组（移植​​瘤未遭受门静脉阻塞）。用多层CT扫描所有兔子。结果：所有40只动物均被纳入最终分析而未死亡。即时组和3周组肿瘤均未生长。 3周龄组左牙质萎缩，肿瘤生长受到抑制。肿瘤的最大直径明显小于阳性对照组（2.55±0.46 vs 3.59±0.37 cm，t = 5.57，P <0.001）。 3-wk组的肝和肺转移发生率低于阳性对照组（分别为10％对40％和90％对100％）。 A，B，C和D组中血管内皮生长因子（VEGF）的表达强度分别为0.10±0.06、0.66±0.21、0.28±0.09和1.48±0.32。试验组A的VEGF表达水平显着低于阴性对照组C（t ＝ 5.07； P ＜0.001）。另外，测试组B中的VEGF表达明显低于阳性对照组D中的VEGF表达（t ＝ 6.38； P ＜0.001）。多层CT扫描显示，Ⅲ级（40％）肝动脉分支显示率明显低于Ⅰ级（70％）和Ⅱ级（100％）（P <0.05）。但不同等级的门静脉显示率无明显差异。即刻组和3周组的肝脏血流量（BF），血容量（BV），平均通过时间（MTT）和血管表面通透性（PS）值均低于对照组，但这些值肝动脉分数（HAF）的增加。 BF和BV之间（r = 0.905，P <0.01），BF和PS之间（r = 0.967，P <0.01），BV和PS之间（r = 0.889，P <0.01）存在显着正相关。 PV和HAF之间存在显着的负相关（r = -0.768，P <0.01），PS和HAF之间存在显着的负相关（r = -0.557，P <0.01）。 BF，BV和PS的值与VEGF呈正相关（r_（BF）= 0.842，r_（BV）= 0.579，r_（PS）= 0.811，P <0.01）。然而，MTT和HAF的值与VEGF表达之间没有显着相关性（r_（MTT）= 0.066，r_（HAF）-0.027）。结论：结扎门静脉左外分支是建立肝VX2肿瘤兔门静脉阻塞模型的理想方法。多层CT在评估门静脉阻塞的效果中起着关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号