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首页> 外文期刊>World Journal of Gastroenterology >Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells
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Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells

机译：通过门静脉移植骨髓间充质干细胞产生的胰岛样细胞逆转糖尿病大鼠的高血糖

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AIM: To study the capacity of bone marrow mesenchymal stem cells (BM-MSCs) trans-differentiating into islet-like cells and to observe the effect of portal vein transplantation of islet-like cells in the treatment of streptozotocin-induced diabetic rat. METHODS: BM-MSCs were isolated from SD rats and induced to differentiate into islet-like cells under defined conditions. Differentiation was evaluated with electron microscopy, RT-PCR, immunofluorescence and flow cytometry. Insulin release after glucose challenge was tested with ELISA. Then allogeneic islet-like cells were transplanted into diabetic rats via portal vein. Blood glucose levels were monitored and islet hormones were detected in the liver and pancreas of the recipient by immunohistochemistry. RESULTS: BM-MSCs were sptieroid adherent monolayers with high CD90, CD29 and very low CD45 expression. Typical islet-like cells clusters were formed after induction. Electron microscopy revealed that secretory granules were densely packed within the cytoplasm of the differentiated cells. The spheroid cells expressed islet related genes and hormones. The insulin-positive cells accounted for 19.8% and mean fluorescence intensity increased by 2.6 fold after induction. The cells secreted a small amount of insulin that was increased 1.5 fold after glucose challenge. After transplantation, islet-like cells could locate in the liver expressing islet hormones and lower the glucose levels of diabetic rats during d 6 to d 20. CONCLUSION: Rat BM-MSCs could be transdiffe-rentiated into islet-like cells in vitro. Portal vein transplantation of islet-like cells could alleviate the hyperglycemia of diabetic rats.
机译：目的：研究骨髓间充质干细胞（BM-MSCs）转分化为胰岛样细胞的能力，观察胰岛样细胞门静脉移植对链脲佐菌素诱导的糖尿病大鼠的治疗作用。方法：从SD大鼠中分离出BM-MSCs，并在一定条件下诱导其分化为胰岛样细胞。用电子显微镜，RT-PCR，免疫荧光和流式细胞术评估分化。用ELISA测试葡萄糖激发后的胰岛素释放。然后将同种异体胰岛样细胞通过门静脉移植到糖尿病大鼠中。通过免疫组织化学监测血糖水平，并在接受者的肝脏和胰腺中检测到胰岛激素。结果：BM-MSCs是类孢子状粘附单层细胞，具有较高的CD90，CD29和非常低的CD45表达。诱导后形成典型的胰岛样细胞簇。电子显微镜显示，分泌颗粒密集地包裹在分化细胞的细胞质内。球状细胞表达了与胰岛相关的基因和激素。诱导后，胰岛素阳性细胞占19.8％，平均荧光强度增加了2.6倍。细胞分泌少量的胰岛素，在葡萄糖激发后增加了1.5倍。移植后，胰岛样细胞可以在表达胰岛激素的肝脏中定位，并在第6天至第20天降低糖尿病大鼠的葡萄糖水平。结论：大鼠BM-MSCs可以体外转分化为胰岛样细胞。胰岛样细胞的门静脉移植可以减轻糖尿病大鼠的高血糖症。

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