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首页> 外文期刊>World Journal of Gastroenterology >Heat-shocked tumor cell lysate-pulsed dendritic cells induce effective anti-tumor immune response in vivo.
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Heat-shocked tumor cell lysate-pulsed dendritic cells induce effective anti-tumor immune response in vivo.

机译：热激肿瘤细胞裂解物脉冲树突状细胞在体内诱导有效的抗肿瘤免疫应答。

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AIM: To study whether heat-shocked tumor cells could enhance the effect of tumor cell lysate-pulsed dendritic cells (DCs) in evoking anti-tumor immune response in vivo. METHODS: Mouse undifferentiated colon cancer cells (CT-26) were heated at 42 degrees Celsius for 1 h and then frozen-thawed. The bone marrow-derived DCs pulsed with heat-shocked CT-26 cell lysate (HSCT-26 DCs) were recruited to immunize syngeneic naive BALB/c mice. The cytotoxic activity of tumor specific cytotoxic T lymphocytes (CTLs) in mouse spleen was evaluated by IFN-enzyme-linked immunospot (ELISpot) and LDH release assay. The immunoprophylactic effects induced by HSCT-26 DCs in mouse colon cancer model were compared to those induced by single CT-26 cell lysate-pulsed DCs (CT-26 DCs) on tumor volume, peritoneal metastasis and survival time of the mice. RESULTS: Heat-treated CT-26 cells showed a higher hsp70 protein expression. Heat-shocked CT-26 cell lysate pulsing elevated the co-stimulatory and MHC-II molecule expression of bonemarrow-derived DCs as well as interleukin-12 p70 secretion. The IFN-gamma secreting CTLs induced by HSCT-26 DCs were significantly more than those induced by CT-26 DCs (P=0.002). The former CTLs' specific cytotoxic activity was higher than the latter CTLs' at a serial E/T ratio of 10:1, 20:1, and 40:1. Mouse colon cancer model showed that the tumor volume of HSCT-26 DC vaccination group was smaller than that of CT-26 DC vaccination group on tumor volume though there was no statistical difference between them (24 mm3 vs 8 mm3, P=0.480). The median survival time of mice immunized with HSCT-26 DCs was longer than that of those immunized with CT-26 DCs (57 d vs 43 d, P=0.0384). CONCLUSION: Heat-shocked tumor cell lysate-pulsed DCs can evoke anti-tumor immune response in vivo effectively and serve as a novel DC-based tumor vaccine.
机译：目的：研究热休克的肿瘤细胞是否可以增强肿瘤细胞裂解物脉冲树突状细胞（DC）在体内引起抗肿瘤免疫应答的作用。方法：将小鼠未分化结肠癌细胞（CT-26）在42摄氏度下加热1小时，然后冻融。募集经热休克的CT-26细胞裂解物（HSCT-26 DC）脉冲刺激的骨髓来源DC，以免疫同种幼稚BALB / c小鼠。通过IFN-酶联免疫斑点（ELISpot）和LDH释放试验评估了小鼠脾脏中肿瘤特异性细胞毒性T淋巴细胞（CTL）的细胞毒性活性。将HSCT-26 DC诱导的小鼠结肠癌模型的免疫预防效果与单个CT-26细胞裂解物脉冲的DC（CT-26 DC）诱导的小鼠肿瘤体积，腹膜转移和存活时间进行了比较。结果：经热处理的CT-26细胞显示出较高的hsp70蛋白表达。热休克的CT-26细胞裂解物脉冲提高了骨髓来源DC的共刺激和MHC-II分子表达以及白介素12 p70的分泌。 HSCT-26 DC诱导的IFN-γ分泌CTL显着高于CT-26 DC诱导的CTL（P = 0.002）。在连续的E / T比为10：1、20：1和40：1时，前CTL的比细胞毒活性高于后CTL。小鼠结肠癌模型显示HSCT-26 DC疫苗接种组的肿瘤体积比CT-26 DC疫苗接种组的肿瘤体积小，尽管两者之间没有统计学差异（24 mm3对8 mm3，P = 0.480）。 HSCT-26 DC免疫小鼠的中位生存时间比CT-26 DC免疫小鼠的中位生存时间更长（57 d vs 43 d，P = 0.0384）。结论：热激肿瘤细胞裂解物脉冲的DC可以有效地引起体内抗肿瘤免疫反应，并可以作为一种新型的基于DC的肿瘤疫苗。

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