Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes.

Li J; Feng ZH; Li GY; Mou DL; Nie QH

首页> 外文期刊>World Journal of Gastroenterology >Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes.

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Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes.

机译：树突状细胞特异性细胞间粘附分子3捕获nonintegrin在人外周血单核细胞产生的树突状细胞上的表达。

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AIM: To generate dendritic cells (DCs) from human peripheral blood and to detect the expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN; CD209) for the further study of DC-SIGN in hepatitis C virus (HCV) transmission. METHODS: Peripheral blood monocytes were isolated from blood of healthy individuals by Ficoll-Hypaque sedimentation and cultured in complete medium containing rhGM-CSF and rhIL-4. Cells were cultured for seven days, with cytokine addition every two days to obtain immature DCs. Characteristics of the cultured cells were observed under light and scanning microscope, and the expression of DC-SIGN was detected by immunofluorescence staining. RESULTS: After seven-day culture, a large number of cells with typical characteristics of DCs appeared. Their characteristics were observed under light and scanning electron microscope. These cells had a variety of cell shapes such as those of bipolar elongate cells, elaborate stellate cells and DCs. DC-SIGN was detected by immunofluorescence staining and its expression level on cultivated dendritic cells was high. CONCLUSION: DCs with a high expression of DC-SIGN can be generated from human peripheral blood monocytes in complete medium containing rhGM-CSF and rhIL-4.

机译：目的：从人外周血中产生树突状细胞（DC），并检测捕获非整联蛋白的树突状细胞特异性细胞间粘附分子3（DC-SIGN； CD209）的表达，以进一步研究DC-SIGN在丙型肝炎病毒（HCV）中的应用）传播。方法：通过Ficoll-Hypaque沉淀法从健康人血液中分离外周血单核细胞，并在含有rhGM-CSF和rhIL-4的完全培养基中培养。培养细胞7天，每两天添加细胞因子以获得未成熟的DC。在光和扫描显微镜下观察培养的细胞的特征，并通过免疫荧光染色检测DC-SIGN的表达。结果：培养7天后，出现了大量具有典型DCs特征的细胞。在光和扫描电子显微镜下观察它们的特性。这些细胞具有多种细胞形状，例如双极细长细胞，复杂的星状细胞和DC。通过免疫荧光染色检测DC-SIGN，其在培养的树突状细胞中的表达水平很高。结论：在含有rhGM-CSF和rhIL-4的完全培养基中，人外周血单核细胞可产生高表达DC-SIGN的DC。

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来源
《World Journal of Gastroenterology》 |2006年第3期|P.453-456|共4页
作者
Li J; Feng ZH; Li GY; Mou DL; Nie QH;
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作者单位

The Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类消化系及腹部疾病;
关键词
Dendritic Cells; DC-specific ICAM-3 grabbing nonintegrin; Peripheral blood; NOS; 树突细胞; 单核细胞;

机译：Dendritic Cells;DC-specific ICAM-3 grabbing nonintegrin;Peripheral blood;NOS;树突细胞;单核细胞;

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1. Cocaine Modulates Dendritic Cell-Specific C Type Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Expression by Dendritic Cells in HIV-1 Patients [J] . Madhavan P. N. Nair, Supriya D. Mahajan, Stanley A. Schwartz, The journal of immunology . 2005,第11期

机译：可卡因调节HIV-1患者树突状细胞特异的树突状细胞特定的C型细胞间粘附分子3抓住非整联蛋白的表达。
2. Cocaine Modulates Dendritic Cell-Specific C Type Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Expression by Dendritic Cells in HIV-1 Patients. [J] . Nair MP, Mahajan SD, Schwartz SA, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2005,第11期

机译：可卡因通过HIV-1患者的树突状细胞调节树突状细胞特异性C型细胞间粘附分子3-捕获非整联蛋白的表达。
3. Expression of dendritic cell lysosome-associated membrane protein and dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin in condyloma acuminatum lesions [J] . Wei-Yuan Ma, Le Zhuang, Qi-Chao Qi, Journal of International Medical Research . 2013,第1期

机译：树突状细胞溶酶体相关膜蛋白和树突状细胞特异性细胞间黏附分子3捕获非整合素在尖锐湿疣中的表达
4. Intercellular adhesion molecule 1-dependent activation of interleukin 8 expression in Candida albicans-infected human gingival epithelial cells [C] . Hiroshi Egusa, Hiroki Nikawa, Seicho Makihir International Symposium for Interface Oral Health Science . 2005

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5. Macrophage colony stimulating factor-induced differentiation of human cord blood monocytes into IL-10(high)IL-12(absent) dendritic cells with tolerogenic potential and into a rare population of CD16(+) monocytes. [D] . Li, Geling. 2005

机译：巨噬细胞集落刺激因子诱导人脐带血单核细胞分化为具有耐受原性的IL-10（高）IL-12（缺失）树突状细胞和稀有的CD16（+）单核细胞。
6. Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes [O] . Jun Li, Zhi-Hua Feng, Guang-Yu Li, 2006

机译：树突状细胞特异性细胞间粘附分子3捕获nonintegrin在人外周血单核细胞产生的树突状细胞上的表达
7. Cocaine Modulates Dendritic Cell-Specific C Type Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Expression by Dendritic Cells in HIV-1 Patients [O] . Madhavan P. N. Nair, Supriya D. Mahajan, Stanley A. Schwartz, 2005

机译：可卡因通过HIV-1患者的树突细胞调节树突细胞特异性C型细胞间粘附分子-3-抓住非抗蛋白表达

Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes.

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