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首页> 外文期刊>World Journal of Gastroenterology >Effect of WeiJia on carbon tetrachloride induced chronic liver injury.
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Effect of WeiJia on carbon tetrachloride induced chronic liver injury.

机译：围甲对四氯化碳诱导的慢性肝损伤的作用。

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AIM: To study the effect of WeiJia on chronic liver injury using carbon tetrachloride (CCl(4)) induced liver injury animal model. METHODS:Wistar rats weighing 180-220g were randomly divided into three groups: normal control group (Group A), CCl(4) induced liver injury control group (Group B) and CCl(4) induction with WeiJia treatment group (Group C). Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCl(4) in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of WeiJia at a dose of 1.25 microg/kg body weight. Animals were sacrificed at the fifth week (4 male, 3 female), and eighth week (4 male, 3 female) respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid (HA), type IV collagen (CIV), gamma-glutamyl transferase (gamma-GT), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Alpha smooth muscle actin (alpha-SMA) and proliferating cell nuclear antigen (PCNA) immunohistochemistry were also performed. RESULTS: CCl(4) induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV, ALT, AST and gamma-GT levels after eight weeks of treatment for Group C rats were 58+/-22 microg/L (P<0.01), 57+/-21 microg/L (P<0.01), 47+/-10 U/L (P<0.01), 139+/-13 U/L (P<0.05) and 52+/-21 U/L (P>0.05) respectively, similar to normal control group (Group A), but significantly different from CCl(4) induced liver injury control group (Group B). An increase in PCNA and decrease in alpha-SMA expression level was also observed. CONCLUSION: WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity.
机译：目的：采用四氯化碳（CCl（4））诱导的肝损伤动物模型，研究魏家对慢性肝损伤的作用。方法：Wistar大鼠体重180-220g，随机分为三组：正常对照组（A组），CCl（4）诱导的肝损伤对照组（B组）和魏佳治疗组（C组）诱导的CCl（4）诱导。 。每组由14只大鼠组成。对于B组和C组大鼠，每周两次皮下注射3％/ kg体重的40％CCl（4）在橄榄油中诱导肝损伤和纤维化，持续8周，而A组大鼠则使用橄榄油。从第三周开始，C组大鼠还每天接受腹膜内注射威家（WeiJia），剂量为1.25微克/千克体重。分别在第五周（4只雄性，3只雌性）和第八周（4只雄性，3只雌性）处死动物。测量纤维化程度，并测定肝纤维化和功能的血清学标志物，包括透明质酸（HA），IV型胶原蛋白（CIV），γ-谷氨酰转移酶（γ-GT），丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST） 。还进行了α平滑肌肌动蛋白（α-SMA）和增殖细胞核抗原（PCNA）的免疫组织化学研究。结果：与A组大鼠相比，CCl（4）诱导导致B组和C组大鼠肝损伤和纤维化发展。与B组大鼠相比，C组大鼠的维佳治疗可以显着减轻纤维化状况。治疗三周后可观察到效果，治疗八周后更明显。 C组大鼠治疗八周后的血清HA，CIV，ALT，AST和γ-GT水平为58 +/- 22 microg / L（P <0.01），57 +/- 21 microg / L（P <0.01）与正常对照组相似，分别为47 +/- 10 U / L（P <0.01），139 +/- 13 U / L（P <0.05）和52 +/- 21 U / L（P> 0.05）。 A组），但与CCl（4）诱导的肝损伤对照组（B组）有显着差异。还观察到PCNA的增加和α-SMA表达水平的降低。结论：胃甲可以通过抑制星状细胞活性来改善肝功能，减少肝纤维化。

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