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首页> 外文期刊>World Journal of Gastroenterology >Establishment of model of visceral pain due to colorectal distension and its behavioral assessment in rats.
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Establishment of model of visceral pain due to colorectal distension and its behavioral assessment in rats.

机译：大鼠大肠扩张引起的内脏痛模型的建立及其行为学评价。

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AIM: To establish a visceral pain model via colorectal distension (CRD) and to evaluate the efficiency of behavioral responses of CRD by measuring the score of abdominal withdrawal reflex (AWR) in rats. METHODS: Thirty-eight male SD rats weighing 180-240 g were used to establish the visceral pain model. The rat was inserted intra-anally with a 7 cm long flexible latex balloon under ether anesthesia, and colorectal distensions by inflating the balloon with air were made 30 min after recovering from the anesthesia. Five AWR scores (AWR0 to AWR4) were used to assess the intensity of noxious visceral stimuli. It was regarded as the threshold of the minimal pressure (kPa). For abdominal flatting was induced by colorectal distension. RESULTS: A vigorous AWR to distension of the descending colon and rectum was found in 100% of the awake rats tested. The higher the pressure of distension, the higher the score of AWR. The distension pressures of 0, 2.00, 3.33, 5.33 and 8.00 kPa produced different AWR scores (P<0.05). The pain threshold of AWR was constant for up to 80 min after the initial windup (first 1-3 distensions), the mean threshold was 3.69+/-0.35 kPa. Systemic administration of morphine sulfate elevated the threshold of visceral pain in a dose-dependent and naloxone reversible manner. CONCLUSION: Scoring the AWR during colorectal distensions can assess the intensity of noxious visceral stimulus. Flatting of abdomen (AWR 3) to CRD as the visceral pain threshold is clear, constant and reliable. This pain model and its behavioral assessment are good for research on visceral pain and analgesics.
机译：目的：通过大肠扩张（CRD）建立内脏疼痛模型，并通过测量大鼠的腹部退缩反射（AWR）评分来评估CRD的行为反应效率。方法：38只体重180-240 g的雄性SD大鼠建立内脏痛模型。在乙醚麻醉下，用7cm长的柔性乳胶球囊将大鼠插入肛门内，并在从麻醉中恢复后30分钟，通过向气球充气来使结肠扩张。五个AWR评分（AWR​​0至AWR4）用于评估有害内脏刺激的强度。它被视为最小压力（kPa）的阈值。对于腹部扁平症是由大肠扩张引起的。结果：在100％的清醒大鼠中发现了强烈的AWR导致结肠和直肠下降。扩张压力越高，AWR得分越高。 0、2.00、3.33、5.33和8.00 kPa的膨胀压力产生不同的AWR评分（P <0.05）。 AWR的疼痛阈值在初始缠绕（最初的1-3次扩张）后的80分钟内保持恒定，平均阈值为3.69 +/- 0.35 kPa。硫酸吗啡的全身给药以剂量依赖性和纳洛酮可逆的方式升高内脏疼痛的阈值。结论：在结直肠扩张期间对AWR评分可以评估有害内脏刺激的强度。由于内脏痛阈值清晰，恒定且可靠，因此腹部扁平化（AWR 3）至CRD。该疼痛模型及其行为评估对内脏痛和止痛药的研究非常有用。

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