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首页> 外文期刊>World Journal of Gastroenterology >Inhibitory effect of caffeic acid phenethyl ester on the growth of SW480 colorectal tumor cells involves beta-catenin associated signaling pathway down-regulation.
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Inhibitory effect of caffeic acid phenethyl ester on the growth of SW480 colorectal tumor cells involves beta-catenin associated signaling pathway down-regulation.

机译：咖啡酸苯乙酯对SW480结直肠肿瘤细胞生长的抑制作用涉及β-catenin相关的信号通路下调。

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摘要

AIM: To study the anti-tumor effect of caffeic acid phenethyl ester (CAPE) and the influence of CAPE on beta-catenin associated signaling pathway in SW480 colorectal cancer (CRC) cells. METHODS: SW480 cells were treated with CAPE at serial concentrations. The proliferative status of cells was measured by methabenzthiazuron (MTT) assay. Cell cycle and cell apoptosis were analyzed using flow cytometry (FCM). Western blotting assay was used to evaluate the protein level of beta-catenin, c-myc and cyclinD1. Beta-catenin localization was determined by indirect immunofluorescence. RESULTS: CAPE displayed a strong inhibitory effect in a significant dose- and time-dependent manner on SW480 cell growth. FCM analysis showed that the ratio of G0/G1 phase cells increased, S phase ratio decreased and apoptosis rate increased after SW480 cells were exposed to CAPE for 24 h. Pretreatment of SW480 cells with CAPE significantly suppressed beta-catenin, c-myc and cyclinD1 protein expression. CAPE treatment was associated with decreased accumulation of beta-catenin protein in nucleus and cytoplasm, and concurrently increased its accumulation on the surface of cell membrane. CONCLUSION: CAPE can inhibit SW480 cell proliferation by inducing cell cycle arrest and apoptosis. Decreased beta-catenin and the associated signaling pathway target gene expression may mediate the anti-tumor effects of CAPE.
机译：目的：研究咖啡酸苯乙酯（CAPE）的抗肿瘤作用以及CAPE对SW480大肠癌细胞（CRC）细胞中β-catenin相关信号通路的影响。方法：以连续浓度的CAPE处理SW480细胞。细胞的增殖状态通过甲苯并噻唑啉酮（MTT）测定来测量。使用流式细胞仪（FCM）分析细胞周期和细胞凋亡。蛋白质印迹法用于评估β-catenin，c-myc和cyclinD1的蛋白水平。 β-连环蛋白的定位是通过间接免疫荧光测定的。结果：CAPE对SW480细胞的生长具有明显的剂量和时间依赖性，并具有很强的抑制作用。流式细胞仪分析表明，SW480细胞暴露于CAPE 24 h后，G0 / G1期细胞比例增加，S期比例降低，凋亡率增加。用CAPE预处理SW480细胞可显着抑制β-catenin，c-myc和cyclinD1蛋白表达。 CAPE处理与减少β-catenin蛋白在细胞核和细胞质中的积累有关，并同时增加其在细胞膜表面的积累。结论：CAPE可通过诱导细胞周期停滞和凋亡来抑制SW480细胞的增殖。 β-catenin和相关信号通路靶基因表达的减少可能介导CAPE的抗肿瘤作用。

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