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首页> 外文期刊>World Journal of Gastroenterology >Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at stomach meridian acupoints.
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Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at stomach meridian acupoints.

机译：表皮生长因子受体基因在胃经络穴位经电针处理的大鼠血清增强在胃粘膜细胞中的表达。

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AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 +/- 0.0594), the serum derived from rats of the stomach group (1.2272 +/- 0.0813, P = 0.00 < 0.01) and gallbladder group (0.9640 +/- 0.0387, P = 0.00 < 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 +/- 0.0457) but with no significant difference (P = 0.495 > 0.05) and in model serum group (0.8516 +/- 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 < 0.01). CONCLUSION: The present study shows that serum derived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory "particular relation between meridian and internal organ".
机译：目的：探讨电针治疗胃经穴的大鼠血清对胃黏膜细胞表皮生长因子受体（EGFR）基因表达的影响。方法：采用水浸和约束应激法建立大鼠应激性胃粘膜损伤模型。 52只大鼠随机分为：正常组（n = 8），模型组（n = 8），模型血清组（n = 12），胃血清组（n = 12）和胆囊血清组（n = 12）。 。通过蛋白酶-EDTA消化法分离胃粘膜细胞，并与血清一起温育。采用逆转录-聚合酶链反应（RT-PCR）方法检测胃黏膜细胞中EGFR基因的表达。结果：与正常组（0.6860 +/- 0.0594）相比，胃组（1.2272 +/- 0.0813，P = 0.00 <0.01）和胆囊组（0.9640 +/- 0.0387，P = 0.00 < 0.01）具有增强胃粘膜细胞中EGFR基因表达的趋势。模型组（0.7104 +/- 0.0457）中存在这种趋势，但差异无统计学意义（P = 0.495> 0.05），模型血清组（0.8516 +/- 0.0409）存在显着差异（P = 0.001 <0.01）。 。此外，胃血清组的EGFR基因表达明显高于胆囊血清组（P = 0.00 <0.01）。结论：本研究表明，电针治疗胃经穴的大鼠血清可明显增加胃粘膜细胞的EGFR基因表达。因此，血清中存在一定的经络特异性，可以为中医理论“经络与内脏之间的特殊关系”提供依据。

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