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首页> 外文期刊>World Journal of Gastroenterology >Expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in ulcerative colitis.
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Expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in ulcerative colitis.

机译：溃疡性结肠炎中基质金属蛋白酶-1和组织蛋白酶-1的表达

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AIM: To examine the expression of metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the colonic mucosa of patients with ulcerative colitis (UC). METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to study the expression of MMP-1 and TIMP-1 at both mRNA and protein levels in patients with UC and controls. The relationship between MMP-1 mRNA, TIMP-1 mRNA, MMP-1 mRNA/TIMP-1 mRNA ratio and the severity of clinical symptoms of the patients with UC were also analyzed. RESULTS: The expression of MMP-1 mRNA and TIMP-1 mRNA in the ulcerated and inflamed colonic mucosa was significantly higher than that in the non-inflamed colonic mucosa (P < 0.001), but there was no statistically significant difference in the non-inflamed colonic mucosa of UC patients and normal controls (P > 0.05). The mRNA expression of MMP-1 and TIMP-1 in ulcerated colonic mucosa of UC patients was increased by 80-fold and 2.2-fold, respectively when comparedwith the normal controls. In the inflamed colonic mucosa, the increase was 30-fold and 1.6-fold, respectively. Immunohistochemical analysis showed that among the ulcerated, inflamed, and non-inflamed colonic mucosae of UC patients and the normal controls, the positive rate of MMP-1 expression was 87%, 87%, 40% and 35% respectively, and the positive rate of TIMP-1 expression was 89%, 89%, 80% and 75%, respectively. Furthermore, the expression of MMP-1 mRNA, TIMP-1 mRNA and the MMP-1 mRNA/ TIMP-1 mRNA ratio were correlated with the severity of clinical symptoms (P <0.05). CONCLUSION: Excessive expression of MMP-1 in the diseased colonic mucosa causes excessive hydrolysis of the extracellular matrix (ECM) and ulceration in UC patients. MMP-1 mRNA, TIMP-1 mRNA and MMP-1 mRNA/TIMP-1 mRNA ratio can be used as biomarkers to judge the severity of clinical symptoms in patients with UC. Exogenous TIMP-1 or MMP-1 inhibitor therapy is a novel treatment for patients with UC.
机译：目的：探讨溃疡性结肠炎（UC）患者结肠黏膜中金属蛋白酶-1（MMP-1）和金属蛋白酶-1组织抑制剂（TIMP-1）的表达。方法：采用逆转录聚合酶链反应（RT-PCR）和免疫组化方法研究UC和对照组UC患者MMP-1和TIMP-1在mRNA和蛋白水平的表达。还分析了UC患者的MMP-1 mRNA，TIMP-1 mRNA，MMP-1 mRNA / TIMP-1 mRNA比率与临床症状严重程度的关系。结果：溃疡性和发炎性结肠黏膜中MMP-1 mRNA和TIMP-1 mRNA的表达明显高于非发炎性结肠黏膜中的表达（P <0.001），但非炎症性结肠黏膜中MMP-1 mRNA和TIMP-1 mRNA的表达无统计学意义。 UC患者和正常对照组的结肠黏膜发炎（P> 0.05）。与正常对照组相比，UC患者溃疡性结肠黏膜中MMP-1和TIMP-1的mRNA表达分别增加了80倍和2.2倍。在发炎的结肠粘膜中，增加分别为30倍和1.6倍。免疫组织化学分析显示，在UC患者和正常对照组的溃疡，发炎和未发炎的结肠黏膜中，MMP-1表达的阳性率分别为87％，87％，40％和35％，阳性率TIMP-1的表达分别为89％，89％，80％和75％。此外，MMP-1 mRNA，TIMP-1 mRNA的表达以及MMP-1 mRNA / TIMP-1 mRNA的比例与临床症状的严重程度相关（P <0.05）。结论：患病的结肠粘膜中MMP-1的过量表达会导致UC患者细胞外基质（ECM）过度水解和溃疡。 MMP-1 mRNA，TIMP-1 mRNA和MMP-1 mRNA / TIMP-1 mRNA的比率可以用作判断UC患者临床症状严重程度的生物标记。外源TIMP-1或MMP-1抑制剂疗法是一种用于UC患者的新型疗法。

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