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首页> 外文期刊>World Journal of Gastroenterology >Total plasma homocysteine and methylenetetrahydrofolate reductase C677T polymorphism in patients with colorectal carcinoma.
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Total plasma homocysteine and methylenetetrahydrofolate reductase C677T polymorphism in patients with colorectal carcinoma.

机译：大肠癌患者血浆总同型半胱氨酸和亚甲基四氢叶酸还原酶C677T多态性。

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摘要

AIM: To investigate the behaviour of total plasma homocysteine (tHcy) and its most common genetic determinant defect, the methylenetetrahydrofolate reductase C677T (C677TMTHFR) polymorphism in patients with early stage colorectal carcinoma. METHODS: tHcy was quantified by Abbott IMx immunoassay; screening for C677TMTHFR substitution was performed by PCR and restriction analysis. RESULTS: The frequency of the C/T and T/T genotypes of the C677TMTHFR gene polymorphism did not differ between the groups. The mean tHcy was statistically higher in cancer patients than in control subjects carrying the same C/C or C/T genotype, whereas there was no difference in the T/T homozygous carriers of the two groups. tHcy was significantly higher in the T/T homozygous carriers than in C/C and C/T genotype carriers. CONCLUSION: The statistically significant increase of tHcy observed in C/C and C/T genotype carriers among our cancer patients is related to substrate consumption dependent on the tumor cell proliferation rate, whereas the tHcy increase observed in T/T genotype carriers of both groups probably depends on the enzymatic deficit of the homocysteine conversion to methionine and/or on the folate deficiency.
机译：目的：研究早期大肠癌患者血浆总同型半胱氨酸（tHcy）的行为及其最常见的遗传决定因素缺陷-亚甲基四氢叶酸还原酶C677T（C677TMTHFR）多态性。方法：通过Abbott IMx免疫测定法定量tHcy。通过PCR和限制分析进行C677TMTHFR取代的筛选。结果：C677TMTHFR基因多态性的C / T和T / T基因型频率在两组之间没有差异。癌症患者的平均tHcy在统计学上高于携带相同C / C或C / T基因型的对照组，而两组的T / T纯合子携带者没有差异。 T / T纯合子携带者的tHcy显着高于C / C和C / T基因型携带者。结论：在我们的癌症患者中，C / C和C / T基因型携带者中观察到的tHcy的统计学显着增加与底物消耗有关，这取决于肿瘤细胞的增殖速率，而两组T / T基因型携带者中观察到的tHcy增加可能取决于高半胱氨酸向蛋氨酸转化的酶促缺陷和/或叶酸缺乏。

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