...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Effects of gastrin 17 on β-catenin/Tcf-4 pathway in Colo320WT colon cancer cells
【24h】

Effects of gastrin 17 on β-catenin/Tcf-4 pathway in Colo320WT colon cancer cells

机译：胃泌素17对Colo320WT结肠癌细胞β-catenin/ Tcf-4途径的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To explore the effect of gastrin 17 (G17) on β-catenin/T cell factor-4 (Tcf-4) signaling in colonic cancer cell line Colo320WT. METHODS: The pCR3.1/GR plasmid, which expresses gastrin receptor, cholecystokinin-2 receptor (CCK-2R), was transfected into a colonic cancer cell line Colo320 by Lipofectamine ™2000 and the stably expressing CCK-2R clones were screened by G418. The expression levels of gastrin receptor in the Colo320 and the transfected Colo320WT cell line were assayed by RT-PCR. Colo320WT cells were treated with G17 in a time-dependent manner (0, 1, 6, 12, 24 and 48 h), then with L365,260 (Gastrin_(17) receptor blocker) for 30 min, and with G17 again for 12 h or L365,260 for 12 h. Expression levels of β-catenin in a TX-100 soluble fraction and TX-100 insoluble fraction of Colo320WT cells treated with G17were detected by co-immuniprecipation and Western blot. Immunocytochemistry was used to examine the distribution of β-catenin in CoLoWT320 cells. Expression levels of c-myc and cyclin D1 in Colo320WT cells treated with G17 were assayed by Western blot. RESULTS: Expression levels of β-catenin in the TX-100 solution fraction decreased apparently in a time-dependent fashion and reached the highest level after G17 treatment for 12 h, while expression levels of β-catenin in the TX-100 insoluble fraction were just on the contrary. Immunocytochemistry showed that β-catenin was translocated from the cell membranes into the cytoplasm and nucleus under G17 treatment. Expression levels of c-myc and cyclin D1 in the G17-treated Colo320WT cells were markedly higher compared to the untreated Colo320WT cells. In addition, the aforementioned G17-stimulated responses were blocked by L365,260. CONCLUSION: Gastrin17 activates β-catenin/Tcf-4 signaling in Colo320WT cells, thereby leading to over-expression of c-myc and cyclin D1.
机译：目的：探讨胃泌素17（G17）对结肠癌细胞系Colo320WT中β-catenin/ T细胞因子-4（Tcf-4）信号传导的影响。方法：用Lipofectamine™2000将表达胃泌素受体胆囊收缩素2受体（CCK-2R）的pCR3.1 / GR质粒转染到结肠癌细胞系Colo320中，并通过G418筛选稳定表达的CCK-2R克隆。 。通过RT-PCR测定胃泌素受体在Colo320和转染的Colo320WT细胞系中的表达水平。将Colo320WT细胞以时间依赖性方式（0、1、6、12、24和48小时）用G17处理，然后用L365,260（Gastrin_（17）受体阻滞剂）处理30分钟，再用G17处理12分钟h或L365,260持续12 h。通过免疫共沉淀和Western印迹检测G17处理的Colo320WT细胞的TX-100可溶部分和TX-100不溶部分中β-catenin的表达水平。免疫细胞化学用于检查CoLoWT320细胞中β-catenin的分布。通过Western印迹测定在用G17处理的Colo320WT细胞中c-myc和细胞周期蛋白D1的表达水平。结果：TX-100溶液级分中β-catenin的表达水平呈时间依赖性降低，经G17处理12 h达到最高水平，而TX-100不溶级分中β-catenin的表达水平最高。恰恰相反。免疫细胞化学显示，在G17处理下，β-catenin从细胞膜转移到细胞质和细胞核中。与未处理的Colo320WT细胞相比，经G17处理的Colo320WT细胞中c-myc和细胞周期蛋白D1的表达水平明显更高。另外，上述G17刺激的应答被L365，260阻断。结论：胃泌素17激活Colo320WT细胞中的β-catenin/ Tcf-4信号传导，从而导致c-myc和cyclin D1的过表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号