• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >H pylori infection and systemic antibodies to CagA and heat shock protein 60 in patients with coronary heart disease.
【24h】

H pylori infection and systemic antibodies to CagA and heat shock protein 60 in patients with coronary heart disease.

机译：冠心病患者的幽门螺杆菌感染以及针对CagA和热休克蛋白60的全身性抗体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: to determine the overall prevalence of H pylori and CagA positive H pylori infection and the prevalence of other bacterial and viral causes of chronic infection in patients with coronary heart disease (CHD), and the potential role of anti-heat-shock protein 60 (Hsp60) antibody response to these proteins in increasing the risk of CHD development. METHODS: Eighty patients with CHD and 160 controls were employed. We also compared the levels of anti-heat-shock protein 60 (Hsp60) antibodies in the two groups. The H pylori infection and the CagA status were determined serologically, using commercially available enzyme-linked immunosorbent assays (ELISA), and a Western blotting method developed in our laboratory. Systemic antibodies to Hsp60 were determined by a sandwich ELISA, using a polyclonal antibody to Hsp60 to sensitise polystyrene plates and a commercially available human Hsp60 as an antigen. RESULTS: The overall prevalence of H pylori infection was 78.7% (n = 63) in patients and 76.2% (n = 122) in controls (P = 0.07). Patients infected by CagA-positive (CagA(+)) H pylori strains were 71.4% (n = 45) vs 52.4% of infected controls (P = 0.030, OR = 2.27). Systemic levels of IgG to Hsp60 were increased in H pylori-negative patients compared with uninfected controls (P < 0.001) and CagA-positive infected patients compared with CagA-positive infected controls (P = 0.007). CONCLUSION: CagA positive H pylori infection may concur to the development of CHD; high levels of anti-Hsp60 antibodies may constitute a marker and/or a concomitant pathogenic factor of the disease.
机译：目的：确定幽门螺杆菌和CagA阳性幽门螺杆菌感染的总体患病率以及冠心病（CHD）患者慢性感染的其他细菌和病毒原因的患病率，以及抗热休克蛋白60的潜在作用（Hsp60）抗体对这些蛋白质的反应增加了CHD发生的风险。方法：80例冠心病患者和160名对照者。我们还比较了两组中的抗热休克蛋白60（Hsp60）抗体的水平。幽门螺杆菌感染和CagA的状态是通过血清学测定的，使用可商购的酶联免疫吸附测定（ELISA）和在我们实验室中开发的Western blotting方法。通过夹心ELISA确定针对Hsp60的全身抗体，使用针对Hsp60的多克隆抗体使聚苯乙烯板敏化并且使用市售人Hsp60作为抗原。结果：幽门螺杆菌感染的总体患病率在患者中为78.7％（n = 63），在对照组中为76.2％（n = 122）（P = 0.07）。受CagA阳性（CagA（+））幽门螺杆菌菌株感染的患者为71.4％（n = 45），而受感染的对照组为52.4％（P = 0.030，OR = 2.27）。与未感染的对照组相比，幽门螺杆菌阴性患者的全身IgG水平对Hsp60的升高（P <0.001），与CagA阳性感染的患者相比，CagA阳性感染的患者（P = 0.007）。结论：CagA阳性幽门螺杆菌感染可能与冠心病的发展有关。高水平的抗Hsp60抗体可能构成该疾病的标志物和/或伴随的致病因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号