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首页> 外文期刊>World Journal of Gastroenterology >Tissue microarray for high-throughput analysis of gene expression profiles in hepatocellular carcinoma.
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Tissue microarray for high-throughput analysis of gene expression profiles in hepatocellular carcinoma.

机译：组织芯片用于肝细胞癌基因表达谱的高通量分析。

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AIM: To study the expression profiles of HBsAg, HBcAg, p21(WAF1/CIP1) (p21), Rb genes in hepatocellular carcinoma (HCC) and to investigate their roles in the hepatocar-cinogenesis. METHODS: HCC tissue microarray containing 120-min tissues of 40 HCC cases was constructed. HBsAg, HBcAg, p21 and Rb proteins were immunohistochemically stained by streptavidin-peroxidase conjugated method (S-P). The expression loss of these genes in cancerous, para-cancerous tissues and adjacent normal liver tissues of 40 HCCs were comparatively examined. RESULTS: The positive rate of HBsAg expression in cancerous tissues of 40 HCCs was 7.5%, which was lower than that in para-cancerous and adjacent normal liver tissues (chi(2) =12.774, P<0.01; chi(2) = 18.442, P<0.01). The positive rate of HBcAg expression in cancerous tissues of 40 HCCs was 20.0%, which was also lower than that in para-cancerous and adjacent normal liver tissues (chi(2) = 9.482, P<0.01; chi(2) = 14.645, P<0.01). p21 protein deletion rate in cancerous tissues of 40 HCCs was 27.5%, which was higher than that in para-cancerous and adjacent normal liver tissues (chi(2) = 7.439, P<0.01; chi(2) = 11.174, P<0.01). p21 protein deletion correlated remarkably with the pathological grade of HCC (chi(2) = 0.072, P<0.05). Rb protein deletion rate in cancerous tissues of 40 HCCs was 42.5%, which was also higher than that in para-cancerous and adjacent normal liver tissues (chi(2) = 10.551, P<0.01; chi(2) = 18.353, P<0.01). Rb protein deletion rate did not correlate remarkably with tumor size or pathological grade of HCC (chi(2) = 0.014, P>0.05; chi(2) = 0.017, P>0.05). CONCLUSION: Expression deletion of HBsAg, HBcAg, p21 and Rb proteins in HCCs may play important roles in the carcinogenesis of HCC. Tissue microarray is an effective high-throughput technique platform for cancer research.
机译：目的：研究HBsAg，HBcAg，p21（WAF1 / CIP1）（p21），Rb基因在肝细胞癌（HCC）中的表达情况，并探讨其在肝癌发生中的作用。方法：构建包含40例HCC患者120分钟组织的HCC组织微阵列。 HBsAg，HBcAg，p21和Rb蛋白通过链霉亲和素-过氧化物酶偶联方法（S-P）进行免疫组织化学染色。比较检查了这些基因在40例HCC癌，癌旁组织和邻近的正常肝组织中的表达损失。结果：40例肝癌组织中HBsAg阳性表达率为7.5％，低于癌旁及癌旁正常肝组织（chi（2）= 12.774，P <0.01； chi（2）= 18.442）。 ，P ＜0.01）。 40例HCC癌组织中HBcAg表达阳性率为20.0％，也低于癌旁及邻近正常肝组织的HBcAg表达阳性率（chi（2）= 9.482，P <0.01; chi（2）= 14.645， P ＜0.01）。 40例肝癌的癌组织中p21蛋白的缺失率为27.5％，高于癌旁及癌旁正常肝组织的p21蛋白缺失率（chi（2）= 7.439，P <0.01; chi（2）= 11.174，P <0.01 ）。 p21蛋白的缺失与肝癌的病理分级显着相关（chi（2）= 0.072，P <0.05）。 40个HCC癌组织中Rb蛋白的缺失率为42.5％，也高于癌旁及邻近正常肝组织中的Rb蛋白缺失率（chi（2）= 10.551，P <0.01; chi（2）= 18.353，P < 0.01）。 Rb蛋白缺失率与HCC的肿瘤大小或病理分级无显着相关性（chi（2）= 0.014，P> 0.05； chi（2）= 0.017，P> 0.05）。结论：肝癌中HBsAg，HBcAg，p21和Rb蛋白的表达缺失可能在肝癌的发生中起重要作用。组织芯片是用于癌症研究的有效的高通量技术平台。

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