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首页> 外文期刊>World Journal of Gastroenterology >Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip.
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Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip.

机译：使用基因芯片鉴定小鼠肝癌细胞系中的淋巴转移相关基因。

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AIM: In order to obtain lymphogenous metastasis-associated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential. METHODS: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip(r) MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics. RESULTS: Out of the 14 500 known genes investigated, 110 (0.8%) were up regulated at least 2(3) fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 2(3) fold. According to the Gene Ontology and TreeView analysis, the 110 genes were further classified into two groups: differential biological process profile and molecular function profile. CONCLUSION: Using high-throughput gene chip method, a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription, chaperone activity, motor activity, protein kinase activity, receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation. Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments. ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which mightbe used as the diagnostic markers and the therapeutic targets for lymphatic metastasis.
机译：目的：为了获得淋巴转移相关基因，我们比较了具有高淋巴转移潜能的小鼠肝癌细胞系Hca-F和具有低淋巴转移潜能的Hca-P的转录谱。方法：从Hca-F和Hca-P细胞中分离总RNA，并合成为双链cDNA。体外转录双链cDNA用生物素标记（即生物素标记的cRNA，用作探针）。分别与Affymetrix GeneChip MOE430A杂交的cRNA探针（包含22 690个转录本，包括14 500个已知小鼠基因和4 371个EST），并通过GeneArray Scanner扫描信号。然后通过生物信息学分析结果。结果：在研究的14500个已知基因中，有110个（0.8％）被上调至少2（3）倍。在总共4 371个EST中，有17个EST（0.4％）（未提供数据）上调了至少2（3）倍。根据基因本体论和TreeView分析，将110个基因进一步分为两组：差异生物学过程谱和分子功能谱。结论：采用高通量基因芯片方法，研究了与血管生成，细胞粘附，信号转导，细胞运动，转运，微管化过程，细胞骨架组织和生物发生，细胞周期，转录，伴侣活性有关的大量基因及其细胞功能。因此，运动活性，蛋白激酶活性，受体结合和蛋白结合可能与淋巴转移有关，应作为进一步研究的潜在对象。选择细胞周期蛋白D1，Fosl1，Hsp47，EGFR和AR以及Cav-1作为转移表型的可能候选基因，需要在以后的实验中对其进行验证。 EST（未提供数据）可能表明与淋巴转移有关的新基因。验证这些基因的功能有助于确定导致淋巴转移的关键或候选基因/途径，可将其用作淋巴转移的诊断标志物和治疗靶标。

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