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首页> 外文期刊>World Journal of Gastroenterology >Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats.
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Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats.

机译：丹参化纤胶囊对大鼠纤维化肝组织基质金属蛋白酶-1和金属蛋白酶-1组织抑制剂的影响。

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AIM: To investigate the effects of Danshao Huaxian (DSHX) capsules, a preparation of traditional Chinese medicine, on the expression of matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the fibrous livers of rats. METHODS: Eighty male Wistar rats were randomly divided into normal control group (group A), CCl(4)-induced hepatic fibrosis group (group B), non-DSHX-treated group (group C), low dose-treated group (group D), and high dose-treated group (group E). Fibrous liver models in rats were induced by subcutaneous injection of CCl(4), oral administration of alcohol and high-lipid/low-protein diet for 8 wk. After the models were established, the rats in groups D and E were orally given a low dose (0.5 g/kg) and a high dose (1.0 g/kg) of DSHX daily for 8 wk, respectively. Then, the liver indexes, serum hyaluronic acid (HA) and alanine aminotransferase (ALT) were examined. The degree of hepatic fibrosis was evaluated by optical microscopy. Hydroxyproline (Hyp) in the urine was determined, and the expression of MMP-1 and TIMP-1 was detected by immunohistochemical techniques. RESULTS: In groups D and E, the liver indexes, levels of serum HA and ALT reduced and development of hepatic fibrosis weakened significantly. The urinary Hyp and expression of MMP-1 in the liver tissues elevated, but the expression of TIMP-1 decreased obviously, as compared to groups B and C. CONCLUSION: DSHX enhances the expression of MMP-1 but decreases that of TIMP-1 in liver tissues of CCl(4)-induced hepatic fibrotic rats, which may result in its elevated activity that contributes to fighting against hepatic fibrosis.
机译：目的：探讨中药丹参化纤胶囊（DSHX）对纤维组织中基质金属蛋白酶-1（MMP-1）和组织金属蛋白酶-1（TIMP-1）表达的影响。大鼠的肝脏。方法：将80只雄性Wistar大鼠随机分为正常对照组（A组），CCl（4）诱导的肝纤维化组（B组），非DSHX治疗组（C组），低剂量治疗组（A组）。 D）和高剂量治疗组（E组）。皮下注射CCl（4），口服酒精和高脂/低蛋白饮食连续8周可诱导大鼠纤维化肝模型。建立模型后，分别向D和E组的大鼠口服低剂量（0.5 g / kg）和高剂量（1.0 g / kg）的DSHX，连续8周。然后，检查肝脏指标，血清透明质酸（HA）和丙氨酸氨基转移酶（ALT）。通过光学显微镜评价肝纤维化程度。测定尿中的羟脯氨酸（Hyp），并通过免疫组化技术检测MMP-1和TIMP-1的表达。结果：D组和E组肝指​​标，血清HA和ALT水平降低，肝纤维化发展明显减弱。与B组和C组相比，肝组织中尿Hyp和MMP-1的表达升高，但TIMP-1的表达明显降低。结论：DSHX增强了MMP-1的表达但降低了TIMP-1的表达CCl（4）诱导的肝纤维化大鼠肝组织中可能会导致其活性升高，从而有助于对抗肝纤维化。

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