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首页> 外文期刊>World Journal of Gastroenterology >DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma.
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DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma.

机译：胃癌中MMP-9，TIMP-2和E-cadherin的DNA倍性分析和表达。

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AIM: To investigate DNA ploidy and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma and to explore the mechanism of invasion and metastasis of gastric carcinoma. METHODS: Immunohistochemical methods were used to detect the expressions of MMP-9, TIMP-2, and E-cadherin in 156 cases, including 99 cases of gastric carcinoma, 16 cases of adjacent noncancerous mucosa, 16 cases of distant metastases and 25 cases of metastatic lymph node (LN) from gastric carcinoma. Flow cytometry DNA ploidy and S-phase fraction (SPF) analysis were performed on 57 cases, including 47 cases of gastric cancer, 6 cases of adjacent noncancerous mucosa, and 4 cases of distant metastatic cancer. RESULTS: The expression of MMP-9 was significantly correlated with Lauren's classification, Borrmann's classification, LN metastasis, tumor metastasis, and TNM stage, as well as depth of invasion (all P<0.05). The positive rate was lower in noncarcinoma than in carcinoma (31.3% vs 66.7%, P<0.01). The expression of TIMP-2 was significantly correlated with Borrmann's classification, LN metastasis, and the depth of invasion (all P<0.05). The expression of E-cadherin was significantly correlated with differentiation, Lauren's classification, Borrmann's classification, and LN metastasis, as well as the depth of invasion (P<0.01 or P<0.05). E-cadherin was less expressed in carcinoma than in noncarcinoma (42.4% vs 87.5%, P<0.01). There was a positive correlation between MMP-9 and TIMP-2 and a negative correlation between MMP-9 and E-cadherin, but no correlation between TIMP-2 and E-cadherin. Also there was a positive correlation between DNA aneuploid rate and differentiation and LN metastasis. SPF that was higher than 15% was positively correlated with tumor size, differentiation and LN metastasis. And there was a significant difference between carcinoma and noncarcinoma in DNA aneuploid rate and SPF. CONCLUSION: With tumor progression and development of heterogeneity, the abnormal expressions of MMP-9, TIMP-2, and E-cadherin or DNA aneuploid rate or high SPF gradually increases, suggesting that they play a crucial role in gastric carcinoma progression.
机译：目的：探讨胃癌组织中DNA的倍性以及MMP-9，TIMP-2和E-cadherin的表达，探讨胃癌侵袭转移的机制。方法：采用免疫组织化学方法检测156例胃癌组织中MMP-9，TIMP-2和E-cadherin的表达，其中胃癌99例，癌旁非黏膜16例，远处转移16例，胃癌25例。胃癌转移性淋巴结（LN）。对57例患者进行了流式细胞术DNA倍性和S期分数（SPF）分析，其中包括47例胃癌，6例邻近非癌性粘膜和4例远处转移癌。结果：MMP-9的表达与Lauren的分类，Borrmann的分类，LN转移，肿瘤转移和TNM分期以及浸润深度显着相关（均P <0.05）。非癌的阳性率低于癌（31.3％vs 66.7％，P <0.01）。 TIMP-2的表达与Borrmann的分类，LN转移和浸润深度显着相关（均P <0.05）。 E-cadherin的表达与分化，Lauren的分类，Borrmann的分类，LN转移以及浸润深度显着相关（P <0.01或P <0.05）。 E-钙粘着蛋白在癌组织中的表达低于非癌组织（42.4％对87.5％，P <0.01）。 MMP-9与TIMP-2之间呈正相关，而MMP-9与E-钙粘蛋白之间呈负相关，而TIMP-2与E-钙粘蛋白之间无相关。 DNA非整倍体率与分化和LN转移之间也存在正相关。高于15％的SPF与肿瘤大小，分化和LN转移呈正相关。 DNA非整倍体率和SPF在癌与非癌之间有显着差异。结论：随着肿瘤的发展和异质性的发展，MMP-9，TIMP-2和E-cadherin的异常表达或DNA非整倍体率或高SPF逐渐升高，提示它们在胃癌的进展中起着至关重要的作用。

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