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首页> 外文期刊>World Journal of Gastroenterology >Effects of adenovirus-mediated human cyclooxygenase-2 antisense RNA on the growth of hepatocellular carcinoma.
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Effects of adenovirus-mediated human cyclooxygenase-2 antisense RNA on the growth of hepatocellular carcinoma.

机译：腺病毒介导的人类环氧合酶2反义RNA对肝细胞癌生长的影响。

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AIM: To investigate the relation between the expression of cyclooxygenase-2 (COX-2) and liver cancer, to construct the recombinant adenovirus encoding human COX-2 antisense RNA, and to explore its effects on liver cancer cell proliferation. METHODS: We studied the expression of COX-2 in 34 cases of hepatocellular carcinoma (HCC) and SMMC7402 and SMMC7721 by immunohistochemical technique. Recombinant adenovirus Ad-AShcox-2 was constructed and transfected into human HCC cell lines SMMC7402 and SMMC7721, and its effects on COX-2 expression, cell apoptosis and cell cycle were analyzed by flow cytometry. Cell proliferation was determined by colony-forming efficiency. RESULTS: We observed COX-2 expression in 82.4% of the HCC and SMMC7402 cells, but no COX-2 expression in SMMC7721 cells. In addition, recombinant adenovirus encoding antisense COX-2 fragment Ad-AShcox-2 was obtained with the titer of 1.06X10(12) PFU/mL. Ad-AShcox-2 could reduce the expression of COX-2 and enhance the percentage of cells in G(1)/G(0) phase in SMMC7402 cell line. The difference of apoptotic index between the Ad-AShcox-2 group and control group was statistically significant (t( control group ) = 32.62 and t( Ad-LacZ ) = 10.93, P<0.001) in SMMC7402 but not in SMMC7721. Similarly, colony-forming rates of SMMC7402 and SMMC7721 cell lines, after the transfer of Ad-AShcox-2, were (2.7+/-0.94)% and (33.6+/-4.24)%, respectively. CONCLUSION: Reduction in the expression of COX-2 can inhibit COX-2 expressing HCC cells.
机译：目的：研究环氧合酶-2（COX-2）的表达与肝癌的关系，构建编码人COX-2反义RNA的重组腺病毒，探讨其对肝癌细胞增殖的影响。方法：采用免疫组织化学技术研究34例肝细胞癌（HCC）及SMMC7402和SMMC7721中COX-2的表达。构建重组腺病毒Ad-AShcox-2并将其转染到人HCC细胞株SMMC7402和SMMC7721中，通过流式细胞术分析其对COX-2表达，细胞凋亡和细胞周期的影响。细胞增殖通过集落形成效率来确定。结果：我们在82.4％的HCC和SMMC7402细胞中观察到COX-2表达，而在SMMC7721细胞中未观察到COX-2表达。另外，获得了编码反义COX-2片段Ad-AShcox-2的重组腺病毒，其效价为1.06X10（12）PFU / mL。 Ad-AShcox-2可以减少COX-2的表达并增加SMMC7402细胞系G（1）/ G（0）期细胞的百分比。在SMMC7402中，Ad-AShcox-2组与对照组之间的凋亡指数差异具有统计学意义（t（对照组）= 32.62，t（Ad-LacZ）= 10.93，P <0.001），而在SMMC7721中则无统计学意义。同样，Ad-AShcox-2转移后，SMMC7402和SMMC7721细胞系的集落形成率分别为（2.7 +/- 0.94）％和（33.6 +/- 4.24）％。结论：减少COX-2表达可抑制表达COX-2的肝癌细胞。

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