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首页> 外文期刊>World Journal of Gastroenterology >Effect of fish oil enriched enteral diet on inflammatory bowel disease tissues in organ culture: differential effects on ulcerative colitis and Crohn's disease.
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Effect of fish oil enriched enteral diet on inflammatory bowel disease tissues in organ culture: differential effects on ulcerative colitis and Crohn's disease.

机译：富含鱼油的肠内饮食对器官培养中炎症性肠疾病组织的影响：对溃疡性结肠炎和克罗恩病的不同影响。

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AIM: To investigate the influence of fish oil enriched enteral diet on intestinal tissues taken from Crohn's disease (CD), ulcerative colitis (UC) and non-inflamed non-IBD control patients in vitro. METHODS: Colonoscopic biopsies from patients with active CD (n = 4), active UC (n = 7), and non-inflamed non-IBD control patients (n = 4) were incubated (three dilutions of 1:20, 1:10, and 1:5) with Waymouth's culture medium and enteral elemental diet (EO28, SHS, Liverpool, UK) modified in the fatty acid composition with fish oil (EF) in an organ culture system for 24 h. In each experimental set-up, incubation with Waymouth's medium alone as control was included. Tissue viability was assessed by adding bromodeoxyuridine (BrdU) to the culture fluid and immunohistochemically staining for BrdU uptake. Cytokine ratio of IL-1ra/IL-1beta (low ratio indicative of inflammation) and production of those cytokines as a percentage of medium control were assayed in the culture supernatant. RESULTS: Incubation of CD-affected tissue with EF (1:20, 1:10, and 1:5) modestly and non-significantly increased IL-1ra/IL-1beta ratio as compared with medium control (CD 39.1+/-16.1; 26.5+/-7.8, 47.1+/-16.8 vs control 13.0+/-2.2), but incubation of UC-affected tissues increased IL-1ra/IL-1beta ratio significantly in all three dilutions (UC 69.1+/-32.2, P<0.05; 76.1+/-36.4, P = 0.05; 84.5+/-37.3, P<0.02; vs control 10.2+/-3.7). Incubation of non-inflamed non-IBD control tissue did not increase the IL-1ra/IL-1beta ratio in any dilution compared to medium control (69.3+/-47.0, 54.1+/-30.6, 79.4+/-34.0 vs control 76.1+/-37.3). Average percentage production of IL-1beta indexed against medium control was significantly less in UC after EF incubation as compared with CD (UC 24.0+/-4.8 vs CD 51.8+/-8.1; P<0.05). Average percentage production of IL-1ra was markedly higher in UC (135.9+/-3.4) than that in control patients (36.5+/-4.3) (P<0.0001). CONCLUSION: IBD tissues, after incubation with elemental diet modified in its fatty acid composition with fish oil, show an increase in IL-1ra/IL-1beta cytokine ratio. This effect of omega-3 fatty acid modulation is significantly more marked in UC compared with CD and is accompanied by both a reduction of IL-1beta and increase of IL-1ra. The positive direct anti-inflammatory effect of elemental diet with fish oil in tissue affected with UC suggests dietary treatment of UC may be possible.
机译：目的：探讨富含鱼油的肠内饮食对克罗恩氏病（CD），溃疡性结肠炎（UC）和非炎症非IBD对照患者的肠道组织的影响。方法：对患有活动性CD（n = 4），活动性UC（n = 7）和非发炎的非IBD对照患者（n = 4）的患者进行结肠镜活检（3倍稀释度为1：20、1：10） ，以及1：5），在器官培养系统中，用鱼油（EF）修饰脂肪酸成分，并加入Waymouth培养基和肠内元素饮食（EO28，SHS，Liverpool，UK）发酵24小时。在每个实验设置中，均包括仅用Waymouth培养基作为对照的孵育。通过向培养液中添加溴脱氧尿苷（BrdU）并通过免疫组化染色检测BrdU摄取来评估组织活力。在培养上清液中测定IL-1ra /IL-1β的细胞因子比率（低比率表示炎症）和那些细胞因子的产生（以培养基对照的百分比计）。结果：与培养基对照组相比，用EF（1：20、1：10和1：5）对CD感染的组织进行温和且不显着增加的IL-1ra / IL-1beta比培养（CD 39.1 +/- 16.1） ； 26.5 +/- 7.8、47.1 +/- 16.8与对照13.0 +/- 2.2），但在所有三种稀释液中，UC感染的组织的孵育均显着提高了IL-1ra / IL-1beta的比率（UC 69.1 +/- 32.2， P ＜0.05； 76.1 +/- 36.4，P ＝ 0.05； 84.5 +/- 37.3，P ＜0.02；相对于对照10.2 +/- 3.7。与培养基对照相比，在任何稀释度下，非炎症非IBD对照组织的孵育均不会增加IL-1ra / IL-1beta的比例（69.3 +/- 47.0、54.1 +/- 30.6、79.4 +/- 34.0与对照76.1 +/- 37.3）。与CD相比，EF孵育后UC中针对培养基对照的IL-1β的平均产生百分率明显低于CD（UC 24.0 +/- 4.8 vs CD 51.8 +/- 8.1； P <0.05）。 UC（135.9 +/- 3.4）的平均IL-1ra产生百分率显着高于对照组（36.5 +/- 4.3）（P <0.0001）。结论：IBD组织与鱼油中脂肪酸组成经过修饰的元素饮食一起温育后，IL-1ra /IL-1β细胞因子比例增加。与CD相比，UC中的omega-3脂肪酸调节作用明显更为明显，并伴有IL-1β的减少和IL-1ra的增加。鱼油对患有UC的组织的基本饮食具有积极的直接抗炎作用，这表明可以对UC进行饮食治疗。

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