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首页> 外文期刊>World Journal of Gastroenterology >Effects of epidermal growth factor and its signal transductior inhibitors on apoptosis in human colorectal cancer cells
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Effects of epidermal growth factor and its signal transductior inhibitors on apoptosis in human colorectal cancer cells

机译：表皮生长因子及其信号转导抑制剂对大肠癌细胞凋亡的影响

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AIM: The study investigated if EGF signaling inhibitors, EGF antibody and tyrphostin 51 (a tyrosine kinase inhibitor), mediated the action of EGF on apoptosis and the expression of EGF receptors and p21 (a cyclin-dependent kinase inhibitor) of human colorectal cancer cells. METHODS: Human colorectal adenocarcinoma cells (SW480) were incubated with 0.6 mL/L dimethyl sulfoxide (DMSO, the control group), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO, 225 ng/mL EGF+2.5μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO, or 225 ng/mL EGF+215 ng/mL (0.8μmol/L) tyrphostin 51 in 0.6 mL/L DMSO. RESULTS: After 48 h incubation, the levels of EGF in medium significantly increased (p<0.05) in the EGF-treated groups. The numbers of apoptotic cells were significantly fewer (P<0.05) in the EGF+EGF antibody and EGF+tyrphostin 51 groups than those in the control and EGF groups after 12 h treatments. The expression of phosphorylated EGF receptors in the EGF, EGF+EGF antibody, and EGF+tyrphostin 51 groups was 176.8%, 62.4%, and 138.1% of the control group, respectively. The expression of p21 protein in the EGF, EGF+EGF antibody, and EGF+tyrphostin 51 groups was 115.7%, 4.8%, and 61.5% of the control group, respectively. CONCLUSION: The data suggest that EGF antibody and tyrphostin 51 can inhibit the action of EGF on apoptosis in human colorectal cancer cells through down-regulation of EGF receptor and p21 expression.
机译：目的：该研究调查了EGF信号抑制剂，EGF抗体和酪氨酸激酶51（酪氨酸激酶抑制剂）是否介导了EGF对人结直肠癌细胞凋亡以及EGF受体和p21受体（细胞周期蛋白依赖性激酶抑制剂）表达的作用。 。方法：将人大肠腺癌细胞（SW480）与0.6 mL / L二甲基亚砜（DMSO，对照组），225 ng / mL（37.5 nmol / L）EGF在0.6 mL / L DMSO，225 ng / mL EGF +中孵育0.6 mL / L DMSO中的2.5μg/ mL（17 nmol / L）EGF抗体，或0.6 mL / L DMSO中的225 ng / mL EGF + 215 ng / mL（0.8μmol/ L）tyrphostin 51。结果：孵育48 h后，EGF处理组的培养基中EGF水平显着增加（p <0.05）。处理12 h后，EGF + EGF抗体和EGF + tyrphostin 51组的凋亡细胞数量明显少于对照组和EGF组（P <0.05）。 EGF，EGF + EGF抗体和EGF + tyrphostin 51组中磷酸化EGF受体的表达分别为对照组的176.8％，62.4％和138.1％。 EGF，EGF + EGF抗体和EGF + tyrphostin 51组中p21蛋白的表达分别为对照组的115.7％，4.8％和61.5％。结论：EGF抗体和tyrphostin 51可通过下调EGF受体和p21表达来抑制EGF对人大肠癌细胞凋亡的作用。

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