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首页> 外文期刊>World Journal of Gastroenterology >Expression of thymidine phosphorylase by macrophages in colorectal cancer tissues
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Expression of thymidine phosphorylase by macrophages in colorectal cancer tissues

机译：巨噬细胞胸腺嘧啶核苷磷酸化酶在结直肠癌组织中的表达

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AIM: To detect the thymidine phosphorylase (dThdPase) expression in human colorectal cancer tissues and cells. METHODS: Forty specimens resected from patients with colorectal cancer were immunohistochemically stained by 654-1, anti-dThdPase monoclonal antibody, PG-M1, anti-macrophage marker CD68 monoclonal antibody. Morphometrical analysis and positive cell counting were performed. In 27 of 40 specimens, dThdPase activity was also assayed by HPLC. Otherwise, the dThdPase level was measured by ELISA in 6 colorectal cancer cell lines, LS174T, Clone A, Colo320, CX-1, Lovo, and MIP101, as well as in 2 macrophage-like cell lines, THP-1 and U937. RESULTS: dThdPase activity was significantly increased in cancer tissues compared with adjacent normal tissue (p<0.01). In immunohistochemical analysis, it was confirmed that most cells expressed dThdPase were the stromal cells surrounding cancer nests or along the invasive margin of cancer. Based on their morphometrical characteristics, we found that most of them were tumor-associated macrophages (TAMs). The number of dThdPase-positive stromal cells was significantly correlated with the number of CD68-positive macrophages (r=0.76,p<0.0001). By ELISA, 18.2 unit/mg and 19.3 unit/mg of dThdPase protein were detected in THP-1 and U937, but only little was detected in 6 colorectal cancer cell lines. CONCLUSION: The present data suggest that dThdPase expression is seldom detected in colorectal carcinoma cells. TAM is the most important source of dThdPase in colorectal cancer tissues.
机译：目的：检测胸腺嘧啶磷酸化酶（dThdPase）在人大肠癌组织和细胞中的表达。方法：对结直肠癌患者切除的40份标本进行654-1，抗dThdPase单克隆抗体，PG-M1，抗巨噬细胞标记CD68单克隆抗体的免疫组织化学染色。进行形态计量分析和阳性细胞计数。在40个样本中的27个中，还通过HPLC测定了dThdPase活性。否则，通过ELISA在6种结直肠癌细胞系LS174T，克隆A，Colo320，CX-1，Lovo和MIP101以及2种巨噬细胞样细胞系THP-1和U937中测量dThdPase水平。结果：与邻近的正常组织相比，癌组织中的dThdPase活性显着增加（p <0.01）。在免疫组织化学分析中，已证实大多数表达dThdPase的细胞是癌巢周围或癌浸润边缘的基质细胞。基于它们的形态特征，我们发现它们大多数是肿瘤相关的巨噬细胞（TAM）。 dThdPase阳性基质细胞数量与CD68阳性巨噬细胞数量显着相关（r = 0.76，p <0.0001）。通过ELISA，在THP-1和U937中检测到18.2单位/ mg和19.3单位/ mg的dThdPase蛋白，但是在6种结直肠癌细胞系中仅检测到很少。结论：目前的数据表明在大肠癌细胞中很少检测到dThdPase表达。 TAM是结直肠癌组织中dThdPase的最重要来源。

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