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首页> 外文期刊>World Journal of Gastroenterology >Structure analysis and expressions of a novel tetratransmembrane protein, lysosoma-associated protein transmembrane 4 β associated with hepatocellular carcinoma
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Structure analysis and expressions of a novel tetratransmembrane protein, lysosoma-associated protein transmembrane 4 β associated with hepatocellular carcinoma

机译：新型四跨膜蛋白，溶酶体相关蛋白跨膜4β与肝细胞癌的结构分析及表达

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摘要

AIM: To analyze the structure and expressions of the protein encoded by an HCC-associated novel gene, lysosome-associated protein transmembrane 4 β (LAPTM4B). METHODS: Primary structure and fundamental characteristics of LAPTM4B protein were analysed with bioinformatics. Expressions of LAPTM4B in HCC tissues and various cell lines were detected using polyclonal antibodies and Western blot. RESULTS: LAPTM4B encoded two isoforms of proteins with molecular masses 35-ku and 24-ku, respectively. The expression level of LAPTM4B-35 protein in HCC tissues was dramatically upregulated and related to the differentiation status of HCC tissues, and it was also high in some cancer cell lines. Computer analysis showed LAPTM4B was an integral membrane protein with four transmembrane domains. LAPTM4B showed relatively high homology to LAPTM4A and LAPTM5 in various species. CONCLUSION: LAPTM4B gene encoded two isoforms of tetratransmembrane proteins, LAPTM4B-35 and LAPTM4B-24. The expression of LAPTM4B-35 protein is upregulated and associated with poor differentiation in human HCC tissues, and also at high levels in some cancer cell lines. LAPTM4B is an original and conserved protein.
机译：目的：分析HCC相关新基因，溶酶体相关蛋白跨膜4β（LAPTM4B）编码的蛋白的结构和表达。方法：采用生物信息学技术分析LAPTM4B蛋白的基本结构和基本特征。使用多克隆抗体和蛋白质印迹检测LAPTM4B在HCC组织和各种细胞系中的表达。结果：LAPTM4B编码两种蛋白质的同工型，分子量分别为35-ku和24-ku。 LAPTM4B-35蛋白在肝癌组织中的表达水平显着上调并与肝癌组织的分化状态有关，在某些癌细胞系中也很高。计算机分析表明，LAPTM4B是具有四个跨膜结构域的完整膜蛋白。在各种物种中，LAPTM4B与LAPTM4A和LAPTM5具有较高的同源性。结论：LAPTM4B基因编码两个跨膜蛋白同工型：LAPTM4B-35和LAPTM4B-24。 LAPTM4B-35蛋白的表达上调并与人HCC组织中的不良分化有关，在某些癌细胞系中也高水平表达。 LAPTM4B是一种原始且保守的蛋白质。

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