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首页> 外文期刊>World Journal of Gastroenterology >Genomic determination of CR1 CD35 density polymorphism on erythrocytes of patients with gallbladder carcinoma
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Genomic determination of CR1 CD35 density polymorphism on erythrocytes of patients with gallbladder carcinoma

机译：胆囊癌患者红细胞CR1 CD35密度多态性的基因组测定

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AIM: To study the changes of quantitative expression, adhering activity and genomic density polymorphism of complement types in erythrocytes (CR1) of patients with gallbladder carcinoma and the related clinical significance. METHODS: Polymerase chain reaction (PCR), Hind Ⅲ. restriction enzyme digestion, quantitative assay of CR1 and adhering activity assay of CR1 in erythrocytes were used. RESULTS: The number and adhering activity of CR1 in patients with gallbladder carcinoma (0.738±0.23, 45.9±5.7) were significantly lower than those in chronic cholecystitis and cholecystolithiasis (1.078±0.21, 55.1+5.9) and healthy controls (1.252±0.31, 64.2±7.4) (P < 0.01). The number and adhering activity of CR1 in patients with chronic cholecystitis and cholecystolithiasis (1.078±0.21, 55.1±5.9) were significantly lower than those in healthy controls (1.252±0.31, 64.2±7.4) (P < 0.05). There was a positive correlation between quantitative expression and adhering activity of CR1 (r = 0.79, P < 0.01). Compared with those on preoperative day (0.738±0.23, 45.4±4.9), the number and adhering activity of CR1 in patients with gallbladder carcinoma decreased greatly on the third postoperative day (0.310±0.25, 31.8±5.1) (P < 0.01), and on the first postoperative week (0.480±0.25, 38.9±5.2) (P < 0.01), but they were increased slightly than those on the preoperative day (P > 0.05). The number and adhering activity of CR1 recovered in the second postoperative week (0.740±0.24, 46.8±5.9) (P < 0.01) and increased greatly in the third postoperative week (0.858±0.35, 52.7±5.8) (P < 0.01) in comparison with those on the preoperative day and in the first postoperative week. The number and adhering activity of CR1 of gallbladder carcinoma patients with infiltrating, adjacent lymphogenous and distant organ metastases were significantly lower than those of gallbladder carcinoma patients without them (P < 0.01). No difference was observed between the patients with gallbladder carcinoma and healthy individuals in the spot mutation rate of CR1 density gene (χ~2 = 0.521, P > 0.05). The distribution of expression was 67.8% in high expression genomic type, 24.8% in moderate expression genomic type, and 7.4% in low expression genomic type. The number and adhering activity of CR1 high expression genomic type gallbladder carcinomas (0.749±0.22, 42.1±6.2) were significantly lower than those of healthy individuals(1.240±0. 29, 63.9±7.2), and were also significantly lower than those of healthy individuals (0.921±0.23, 54.8±7.1), but no difference was observed between the number and adhering activity of CR1 lower expression genomic type gallbladder carcinomas (0.582±0.18, 44.3±5.5) and those of healthy individuals (0.610±0.20, 45.8±5.7) (P > 0.05). CONCLUSION: Defective expression of CR1 in gallbladder carcinoma is mostly acquired through central peripheral mechanisms. The changes in CR1 quantitative expression and adhering activity are consanguineously related to the development and metastasis in gallbladder carcinoma.
机译：目的：研究胆囊癌患者红细胞（CR1）中补体类型的定量表达，黏附活性和基因组密度多态性的变化及其临床意义。方法：聚合酶链反应（PCR），HindⅢ。用限制性内切酶消化法，CR1定量法和CR1在红细胞中的粘附活性法进行测定。结果：胆囊癌患者CR1的数量和粘附活性（0.738±0.23，45.9±5.7）显着低于慢性胆囊炎和胆囊结石症（1.078±0.21，55.1 + 5.9）和健康对照组（1.252±0.31）， 64.2±7.4）（P <0.01）。慢性胆囊炎和胆囊结石症患者的CR1数量和粘附活性（1.078±0.21，55.1±5.9）显着低于健康对照组（1.252±0.31，64.2±7.4）（P <0.05）。 CR1的定量表达与粘附活性之间呈正相关（r = 0.79，P <0.01）。与术前相比（0.738±0.23，45.4±4.9），胆囊癌患者术后第3天CR1的数量和粘附活性明显降低（0.310±0.25，31.8±5.1）（P <0.01），术后第一周（0.480±0.25，38.9±5.2）（P <0.01），但比术前增加了一点（P> 0.05）。术后第二周CR1的数量和粘附活性（0.740±0.24,46.8±5.9）（P <0.01），术后第三周明显增加（0.858±0.35,52.7±5.8）（P <0.01）。与术前和术后第一周进行比较。浸润，邻近淋巴结和远处器官转移的胆囊癌患者的CR1的数量和粘附活性显着低于没有转移的胆囊癌患者（P <0.01）。胆囊癌患者与健康个体CR1密度基因的点突变率差异无统计学意义（χ〜2 = 0.521，P> 0.05）。高表达基因组类型的表达分布为67.8％，中表达基因组类型的表达分布为24.8％，低表达基因组类型的表达分布为7.4％。 CR1高表达基因组型胆囊癌的数目和粘附活性（0.749±0.22，42.1±6.2）显着低于健康个体（1.240±0。29，63.9±7.2），也显着低于健康个体。健康个体（0.921±0.23，54.8±7.1），但CR1低表达基因型胆囊癌的数目和粘附活性（0.582±0.18，44.3±5.5）与健康个体（0.610±0.20， 45.8±5.7）（P> 0.05）。结论：CR1在胆囊癌中的缺陷表达主要是通过中央外周机制获得的。 CR1定量表达和粘附活性的变化与胆囊癌的发展和转移密切相关。

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