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Effect of hepatoma H22 on lymphatic endothelium in vitro

机译：H22肝癌对淋巴管内皮细胞的影响

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AIM: To determine the effect of metastatic hepatoma cells on lymphangioma-derived endothelium, and to establish in vitro model systems for assessing metastasis-related response of lymphatic endothelium. METHODS: Benign lymphangioma, induced by intraperitoneal injection of the incomplete Freund's adjuvant in BALB/c mice, was embedded in fibrin gel or digested and then cultured in the conditioned medium derived from hepatoma H22. Light and electron microscopy, and the transwell migration assay were used to determine the effect of H22 on tissue or cell culture. Expressions of Flt-4, c-Fos, proliferating cell nuclear antigen (PCNA), and inducible nitric oxide synthase (iNOS) in cultured cells, and content of nitric oxide in culture medium were also examined. RESULTS: The embedded lymphangioma pieces gave rise to array of capillaries, while separated cells from lymphangioma grew to a cobblestone-like monolayer. H22 activated growth and migration of the capillaries and cells, induced expressions of Flt-4, c-Fos, PCNA and iNOS in cultured cells, and significantly increased the content of NO in the culture medium. CONCLUSION: Lymphangioma-derived cells keep the differentiated phenotypes of lymphatic endothelium, and the models established in this study are feasible for in vitro study of metastasis-related response of lymphatic endothelium.
机译：目的：确定转移性肝癌细胞对淋巴管瘤来源的内皮的影响，并建立评估淋巴管内皮转移相关反应的体外模型系统。方法：将腹膜内注射不完全弗氏佐剂诱导的BALB / c小鼠良性淋巴管瘤包埋在纤维蛋白凝胶中或消化，然后在肝癌H22衍生的条件培养基中培养。使用光镜和电子显微镜以及transwell迁移测定法来确定H22对组织或细胞培养的影响。还检查了培养细胞中Flt-4，c-Fos，增殖细胞核抗原（PCNA）和诱导型一氧化氮合酶（iNOS）的表达以及培养基中一氧化氮的含量。结果：包埋的淋巴管瘤碎片引起毛细血管排列，而淋巴管瘤分离的细胞长成鹅卵石样单层。 H22激活毛细血管和细胞的生长和迁移，诱导Flt-4，c-Fos，PCNA和iNOS在培养细胞中的表达，并显着增加培养基中NO的含量。结论：淋巴管瘤来源的细胞保持了淋巴管内皮细胞的分化表型，本研究建立的模型对于体外研究淋巴管内皮转移相关反应具有可行性。

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