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首页> 外文期刊>World Journal of Gastroenterology >Effect of arsenic trioxide on human hepatocarcinoma in nude mice
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Effect of arsenic trioxide on human hepatocarcinoma in nude mice

机译：三氧化二砷对裸鼠人肝癌的影响

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AIM: To study the effect of arsenic trioxide (As_2O_3) on human hepatoma cell line BEL-7402 in vivo. METHODS: Human hepatoma cell line BEL-7402 cultured in vitro was inoculated into nude mice and arsenic trioxide, 5-Fu and saline were injected into abdominal cavity of the nude mice respectively. The volumes of tumor and general conditions of the nude mice and structural changes of the liver and kidney were observed. Morphologic changes were studied under electron microscope. Expression of AFP was investigated by immunohistochemical method. RESULTS: As_2O_3 could inhibit the growth of tumor. The tumor growth inhibitory rate in mice treated with 2.5 mg/kg As_2O_3 was 53.42% on the tenth day. The tumor growth inhibitory rate in mice treated with 5 mg/kg As_2O_3 was 79.28% on the fifth day and 96.58% on the tenth day respectively. As_2O_3 did not damage the liver and kidney of nude mice, or affect the blood system. Typical apoptotic morphological changes were found under electron microscope, and the change of mitochondria was obvious. The expression rate of AFP declined after treatment. CONCLUSION: Arsenic trioxide can induce apoptosis of human hepatoma cells, and inhibit proliferation of tumor with no obvious side effects on liver and kidney.
机译：目的：研究三氧化二砷（As_2O_3）对人肝癌细胞BEL-7402的体内作用。方法：将体外培养的人肝癌BEL-7402细胞接种于裸鼠体内，分别向裸鼠腹腔注射三氧化二砷，5-Fu和生理盐水。观察裸鼠的肿瘤体积和一般状况以及肝和肾的结构变化。在电子显微镜下研究形态变化。通过免疫组织化学方法研究AFP的表达。结果：As_2O_3可以抑制肿瘤的生长。在第10天用2.5mg / kg As_2O_3治疗的小鼠中的肿瘤生长抑制率为53.42％。用5mg / kg As_2O_3治疗的小鼠在第五天的肿瘤生长抑制率分别为79.28％和第十天为96.58％。 As_2O_3不会损害裸鼠的肝脏和肾脏，也不会影响血液系统。电镜观察发现典型的凋亡形态学变化，线粒体变化明显。治疗后AFP的表达率下降。结论：三氧化二砷可诱导人肝癌细胞凋亡，抑制肿瘤的增殖，对肝肾无明显副作用。

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