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Gene expression profiles of hepatoma cell line HLE

机译：肝癌细胞系HLE的基因表达谱

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AIM: To investigate the global gene expression of cancer related genes in hepatoma cell line HLE using Atlas Human Cancer Array membranes with 588 well-characterized human genes related with cancer and tumor biology. METHODS: Hybridization of cDNA blotting membrane was performed with ~(32)P-labeled cDNA probes synthesized from RNA isolated from Human hepatoma cell line HLE and non-cirrhotic normal liver which was liver transplantation donor. AtlaszImage, a software specific to array, was used to analyze the result. The expression pattern of some genes identified by Atlas arrays hybridization was confirmed by reverse transcription polymerase chain reaction (RT-PCR) in 24 pairs of specimens and Northern blot of 4 pairs of specimens. RESULTS: The differential expression of cell cycle/growth regulator in hepatocellular carcinoma (HCC) showed a stronger tendency toward cell proliferation with more than 1.5-fold up-regulation of Cyclin C, ERK5, ERK6, E2F-3, TFDP-2 and CK4. The anti-apoptotic factors such as Akt-1 were up-regulated, whereas the promotive genes of apoptosis such as ABL2 were down-regulated. Among oncogene/ tumors suppressors, SKY was down-regulated. Some genes such as Integrin beta 8, Integrin beta 7, DNA-PK, CSPCP, byglycan, Tenacin and DNA Topo were up-regulated. A number of genes, including LAR, MEK1, eps1 5, TDGF1, ARHGDIA were down-regulated. In general, expression of the cancer progression genes was up-regulated, while expression of anti-cancer progression genes was down-regulated. These differentially expressed genes tested with RT-PCR were in consistent with cDNA array findings. CONCLUSION: Investigation of these genes in HCC is helpful in disclosing molecular mechanism of pathogenesis and progression of HCC. For the first time few genes were discovered in HCC. Further study is required for the precise relationship between the altered genes and their correlation with the pathogenesis of HCC.
机译：目的：使用Atlas人类癌症阵列膜和588个与癌症和肿瘤生物学相关的人类基因，研究肝癌细胞系HLE中癌症相关基因的全球基因表达。方法：用〜（32）P标记的cDNA探针与cDNA印迹膜杂交，该探针是从人肝癌细胞系HLE和非肝硬化正常肝脏（其为肝移植供体）中分离的RNA合成的。 AtlaszImage是阵列专用的软件，用于分析结果。通过逆转录聚合酶链反应（RT-PCR）在24对样品中进行了Atlas阵列杂交鉴定的某些基因的表达模式，并在4对样品中进行了Northern印迹证实。结果：肝细胞癌（HCC）中细胞周期/生长调节剂的差异表达显示出更强的细胞增殖趋势，其中Cyclin C，ERK5，ERK6，E2F-3，TFDP-2和CK4的上调超过1.5倍。抗凋亡因子如Akt-1被上调，而凋亡促进基因如ABL2被下调。在癌基因/肿瘤抑制物中，SKY被下调。一些基因例如整合素β8，整合素β7，DNA-PK，CSPCP，糖聚糖，腱糖和DNA Topo被上调。下调了许多基因，包括LAR，MEK1，eps1、5，TDGF1和ARHGDIA。通常，癌症进展基因的表达被上调，而抗癌进展基因的表达被下调。用RT-PCR测试的这些差异表达基因与cDNA阵列发现一致。结论：对肝癌中这些基因的研究有助于揭示肝癌发病机理和进展的分子机制。第一次在肝癌中发现了很少的基因。改变的基因之间的精确关系及其与肝癌发病机制的相关性需要进一步的研究。

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