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首页> 外文期刊>World Journal of Gastroenterology >Effect of transforming growth factor beta and bone morphogenetic proteins on rat hepatic stellate cell proliferation and trans-differentiation
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Effect of transforming growth factor beta and bone morphogenetic proteins on rat hepatic stellate cell proliferation and trans-differentiation

机译：转化生长因子β和骨形态发生蛋白对大鼠肝星状细胞增殖和转分化的影响

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摘要

AIM: To explore different roles of TGF-β (transforming growth factor beta) and bone morphogenetic proteins (BMPs) in hepatic stellate cell proliferation and trans-differentiation. METHODS: Hepatic stellate cells were isolated from male Sprague-Dawley rats. Sub-cultured hepatic stellate cells were employed for cell proliferation assay with WST-1 reagent and Western blot analysis with antibody against smooth muscle alpha actin (SMA). RESULTS: The results indicated that TGF-β1 significantly inhibited cell proliferation at concentration as low as 0.1 ng/ ml, but both BMP-2 and BMP-4 did not affect cell proliferation at concentration as high as 10 ng/ml. The effect on hepatic stellate cell trans-differentiation was similar between TGF-β1 and BMPs. However, BMPs was more potent at trans-differentiation of hepatic stellate cells than TGF-β1. In addition, we observed that TGF-β1 transient reduced the abundance of SMA in hepatic stellate cells. CONCLUSION: TGF-β may be more important in regulation of hepatic stellate cell proliferation while BMPs may be the major cytokines regulating hepatic stellate cell trans-differentiation.
机译：目的：探讨转化生长因子β（TGF-β）和骨形态发生蛋白（BMPs）在肝星状细胞增殖和转分化中的不同作用。方法：从雄性Sprague-Dawley大鼠中分离出肝星状细胞。将亚培养的肝星状细胞用于WST-1试剂的细胞增殖测定和抗平滑肌α肌动蛋白（SMA）抗体的蛋白质印迹分析。结果：TGF-β1在低至0.1 ng / ml的浓度下能显着抑制细胞增殖，而BMP-2和BMP-4在高至10 ng / ml的浓度下均不影响细胞增殖。 TGF-β1和BMP对肝星状细胞转分化的影响相似。然而，BMPs在肝星状细胞的转分化中比TGF-β1更有效。此外，我们观察到TGF-β1瞬时减少了肝星状细胞中SMA的丰度。结论：TGF-β可能在调节肝星状细胞的增殖中更为重要，而BMPs可能是调节肝星状细胞转分化的主要细胞因子。

著录项

  • 来源
    《World Journal of Gastroenterology》 |2003年第4期|p.784-787|共4页
  • 作者

    Hong Shen; Guo-Jiang Huang; Yue-Wen Gong;

  • 作者单位

    Departments of Internal Medicine, Biochemistry and Medical Genetics, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 消化系及腹部疾病;
  • 关键词

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