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首页> 外文期刊>World Journal of Gastroenterology >Leptin receptor expression in the basolateral nucleus of amygdala of conditioned taste aversion rats
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Leptin receptor expression in the basolateral nucleus of amygdala of conditioned taste aversion rats

机译：条件化厌恶大鼠杏仁核基底外侧核中瘦素受体的表达

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AIM: To determine whether serum leptin level and the leptin receptor (OB-R) expression in the basolateral amygdala (BLA) change following conditioned taste aversion (CTA) formation. METHODS: The serum leptin concentration was measured by rat leptin RIA kit, long and short forms of leptin receptor (OB-Rb and OB-Ra) mRNA in the brain sections were examined by in situ hybridization (ISH) and the expression of OB-R was assessed by immunohistochemistry ABC method with a highly specific goat anti-OB-R antibody. RESULTS: The level of serum leptin didn't show significant difference between CTA and control group. Comparing with the control group, the CTA group had an increase on count of OB-R immunohistochemistry positive-stained cells in the BLA (127+-12 vs 48+-9 per 1 mm~2). The OB-Rb mRNA expression level enhanced by 11.9 % in the BLA, while OB-Ra mRNA level increased by 7.4 % on the choroid plexus in CTA group. So BLA was supposed to be a region where interactions between gustatory and vagal signals take place. CONCLUSION: BLA is one of the sites, which are responsible for CTA formation in the brain. Leptin and OB-R maybe involved in neuronal communication for CTA. So leptin and its receptors probably take part in CTA and integration of autonomic and extroceptive information.
机译：目的：确定在条件性厌恶（CTA）形成后，基底外侧杏仁核（BLA）中的血清瘦素水平和瘦素受体（OB-R）表达是否发生变化。方法：用大鼠瘦素RIA试剂盒测定血清瘦素浓度，通过原位杂交（ISH）检测脑组织中瘦素受体（OB-Rb和OB-Ra）mRNA的长短形式及OB-的表达。通过免疫组织化学ABC方法用高度特异性的山羊抗OB-R抗体评估R。结果：CTA组与对照组的血清瘦素水平无明显差异。与对照组相比，CTA组的BLA中OB-R免疫组织化学阳性染色的细胞数量增加（每1 mm〜2 127 + -12 vs 48 + -9）。 CTA组中，BLA中的OB-Rb mRNA表达水平提高了11.9％，而脉络丛中的OB-Ra mRNA表达水平提高了7.4％。因此，BLA被认为是味觉和迷走神经信号之间相互作用的区域。结论：BLA是其中之一，其负责大脑中CTA的形成。瘦素和OB-R可能参与了CTA的神经元沟通。因此，瘦素及其受体可能参与了CTA并整合了自主和外在信息。

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