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首页> 外文期刊>World Journal of Gastroenterology >Overexpression of Caspase-1 in adenocarcinoma of pancreas and chronic pancreatitis
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Overexpression of Caspase-1 in adenocarcinoma of pancreas and chronic pancreatitis

机译：Caspase-1在胰腺癌和慢性胰腺炎中的过度表达

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AIM: To identify the expression of Caspase-1(interleukin-1β converting enzyme) and its role in adenoma of the pancreas and chronic pancreatitis. METHODS: The expression of Caspase-1 was assessed in 42 pancreatic cancer tissue samples, 38 chronic pancreatitis specimens, and 9 normal pancreatic tissues by immunohistochemistry and Western blot analysis. RESULTS: Overexpression of Caspase-1 was observed in both disorders, but there were differences in the expression patterns in distinct morphologic compartments. Pancreatic cancer tissues showed a clear cytoplasmatic Overexpression of Caspase-1 in tumor cells of 71 % of the tumors, whereas normal pancreatic tissues showed only occasional immunoreactivity. In chronic pancreatitis, Overexpression of Caspase-1 was found in atrophic acinar cells (89 %), hyperplastic ducts (87 %), and dedifferentiating acinar cells (84 %). Although in atrophic cells a clear nuclear expression was found, hyperplastic ducts and dedifferentiating acinar cells showed clear cytoplasmic expression. Western blot analysis revealed a marked expression of the 45 kDa precursor of Caspase-1 in pancreatic cancer and chronic pancreatitis (80 % and 86 %, respectively). Clear bands at 30 kDa, which suggested the plO-p20 heterodimer of active Caspase-1, were found in 60 % of the cancer tissue and 14 % of the pancreatitis tissue specimens, but not in normal pancreatic tissues. CONCLUSION: Overexpression of Caspase-1 is a frequent event in pancreatic disorders and its differential 'expression patterns may reflect two functions of the protease. One is its participation in the apoptotic pathway in atrophic acinar cells and tumor-surrounding pancreatitis tissue, the other is its possible role in proliferative processes in pancreatic cancer cells and hyperplastic duct cells and dedifferentiating acinar cells in chronic pancreatitis.
机译：目的：确定Caspase-1（白介素-1β转化酶）的表达及其在胰腺腺瘤和慢性胰腺炎中的作用。方法：采用免疫组织化学和Western blot方法检测42例胰腺癌组织，38例慢性胰腺炎和9例正常胰腺组织中Caspase-1的表达。结果：在两种疾病中均观察到Caspase-1的过表达，但在不同的形态学区室中表达模式存在差异。胰腺癌组织在71％的肿瘤细胞中显示出明显的胞浆Caspase-1过表达，而正常胰腺组织仅偶发免疫反应。在慢性胰腺炎中，在萎缩性腺泡细胞（89％），增生导管（87％）和去分化腺泡细胞（84％）中发现了Caspase-1的过表达。尽管在萎缩细胞中发现了清晰的核表达，增生性导管和去分化的腺泡细胞显示了清晰的胞质表达。蛋白质印迹分析显示，在胰腺癌和慢性胰腺炎中Caspase-1的45 kDa前体表达显着（分别为80％和86％）。 30 kDa的清晰条带表明有活性Caspase-1的p10-p20异二聚体，在60％的癌组织和14％的胰腺炎组织标本中发现，但在正常的胰腺组织中未发现。结论：Caspase-1的过表达在胰腺疾病中很常见，其差异表达模式可能反映了该蛋白酶的两种功能。一种是它参与萎缩性腺泡细胞和肿瘤周围的胰腺炎组织的凋亡途径，另一种可能是它在胰腺癌细胞和增生性导管细胞的增殖过程中以及慢性胰腺炎的腺泡细胞去分化中的可能作用。

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