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首页> 外文期刊>World Journal of Gastroenterology >Impact of endoscopically minimal involvement on IL-8 mRNA expression in esophageal mucosa of patients with non-erosive reflux disease
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Impact of endoscopically minimal involvement on IL-8 mRNA expression in esophageal mucosa of patients with non-erosive reflux disease

机译：内镜最小化对非糜烂性反流患者食管黏膜IL-8 mRNA表达的影响

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AIM: Little has been known about the pathogenesis of non-erosive reflux disease (NERD). Recent studies have implicated interleukin 8 (IL-8) in the development and progression of gastroesophgeal reflux disease (GERD). The purpose of this study was to determine IL-8 RNA expression levels in NERD patients with or without subtle mucosal changes. METHODS: We studied 26 patients with NERD and 13 asymptomatic controls. Biopsy sample was taken from the esophagus 3 cm above the gastroesophageal junction and snap frozen for measurement of IL-8 mRNA levels by real-time quantitative polymerase chain reaction (PCR). We also examined mRNA expression of IL-8 receptors, CXCR-1 and -2 by reverse transcriptase PCR. The patients were endoscopically classified into grade M (mucosal color changes without visible mucosal break) and N (neither minimal involvement nor mucosal break) of the modified Los Angeles classification. RESULTS: The relative IL-8 mRNA expression levels were significantly higher in esophageal mucosa of NERD patients than those in esophageal mucosa of the controls. There was a significant difference in IL-8 mRNA levels between grades M and N. The CXCR-1 and -2 mRNAs were constitutively expressed in esophageal mucosa. CONCLUSION: Our results suggest that high IL-8 levels in esophageal mucosa may be involved in the pathogenesis of NERD through interaction with its receptors. NERD seems to be composed of a heterogeneous population in terms of not only endoscopically minimal involvement but also immune and inflammatory processes.
机译：目的：关于非侵蚀性反流病（NERD）的发病机理知之甚少。最近的研究已将白介素8（IL-8）牵涉到胃食管反流病（GERD）的发生和发展。这项研究的目的是确定有无粘膜改变的NERD患者中IL-8 RNA表达水平。方法：我们研究了26例NERD患者和13例无症状对照。从胃食管交界处上方3 cm的食道取活检样品，并速冻以通过实时定量聚合酶链反应（PCR）测量IL-8 mRNA水平。我们还通过逆转录酶PCR检测了IL-8受体，CXCR-1和-2的mRNA表达。内镜下将患者分为改良洛杉矶分类的M级（粘膜颜色变化，无可见的粘膜破裂）和N级（既无最小累及也无粘膜破裂）。结果：NERD患者食管黏膜相对IL-8 mRNA表达水平明显高于对照组食管黏膜。在M级和N级之间，IL-8 mRNA水平存在显着差异。CXCR-1和-2 mRNA在食管粘膜中组成性表达。结论：我们的研究结果表明，食管粘膜中高水平的IL-8可能通过与NERD受体的相互作用而参与了NERD的发病机制。 NERD似乎由异质性种群组成，不仅在内窥镜上受累最少，而且免疫和炎症过程也是如此。

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