Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3

Feng-Tian He; Yong-Zhan Nie; Bao-Jun Chen

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Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3

机译：单克隆抗体MC3的重组可溶性单链可变片段的表达与鉴定

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Conclusion: The soluble ScFv of MC3 is successfully produced, which not only provides a possible novel targeting vehicle for in vivo and in vitro study on associated cancers, but also offers the anuibody a stable genetic source. Aim: To generate soluble single chain variable fragments (ScFv)of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. Methods: mRNA was isolated from the hypbridoma cell line Producing MC3 and the DNAs encoding variable domains of Heavy and light chains(VH and VL)of the antibody were Amplified separately by RT-PCR and assembled into ScFv DNA with a linker DNA.

机译：结论：成功制备了MC3的可溶性ScFv，不仅为体内和体外研究相关癌症提供了可能的新型靶向载体，而且为anuibody提供了稳定的遗传来源。目的：产生识别结直肠癌和胃癌的单克隆抗体MC3的可溶性单链可变片段（ScFv）。方法：从hybrididoma细胞株MC3中分离mRNA，通过RT-PCR分别扩增抗体重链和轻链可变区（VH和VL）的DNA，并用接头DNA组装成ScFv DNA。

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《World Journal of Gastroenterology》 |2002年第2期|p.258-262|共5页
作者
Feng-Tian He; Yong-Zhan Nie; Bao-Jun Chen;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类消化系及腹部疾病;
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1. Screening and Identification of Recombinant Anti-Idiotype Antibodies against Gastric Cancer and Colon Cancer Monoclonal Antibodies by a Phage-Displayed Single-Chain Variable Fragment Library [J] . Li ZK, Guo CC, Nie YZ, Journal of biomolecular screening: The official journal of the Society for Biomolecular Screening . 2010,第3期

机译：通过噬菌体展示的单链可变片段文库筛选和鉴定针对胃癌和结肠癌单克隆抗体的重组抗独特型抗体
2. Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody [J] . Lamberski JA, Thompson NE, Burgess RR Protein Expression and Purification . 2006,第1期

机译：源自多元醇反应性单克隆抗体的单链可变片段抗体的表达和纯化
3. CLONING AND EXPRESSION IN ESCHERICHIA COLI OF AN ANTI-CYCLOHEXANEDIONE SINGLE-CHAIN VARIABLE ANTIBODY FRAGMENT AND COMPARISON TO THE PARENT MONOCLONAL ANTIBODY [J] . Webb SR., Hall JC., Lee H. Journal of Agricultural and Food Chemistry . 1997,第2期

机译：环己二酮单链可变抗体片段在大肠杆菌中的克隆和表达及其与父母单克隆抗体的比较
4. Increasing the solubility of single-chain variable fragment (scFv) antibody produced in recombinant Escherichia coli by fed-batch culture with DO-stat. [C] . Nghia N.H., Thu N.P.A., Kishimoto M. 化学工学会研究講演発表会講演要旨集 . 2019

机译：通过用DO-STAT通过FED分批培养增加重组大肠杆菌中的单链可变片段（SCFV）抗体的溶解度。
5. Selection and expression of recombinant antibody fragments against two hydrophobic haptens. [D] . Makvandi-Nejad, Shokouh. 2010

机译：针对两个疏水性半抗原的重组抗体片段的选择和表达。
6. Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3 [O] . Feng-Tian He, Yong-Zhan Nie, Bao-Jun Chen, 2002

机译：单克隆抗体MC3的重组可溶性单链可变片段的表达与鉴定
7. Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3 [O] . Feng-Tian He, Yong-Zhan Nie, Bao-Jun Chen, 2002

机译：单克隆抗体MC3重组可溶性单链可变片段的表达及鉴定

Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3

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