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首页> 外文期刊>World Journal of Gastroenterology >Salvia miltiorrhiza monomer IH764-3 induces hepatic stellate cell apoptosis via caspase-3 activation
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Salvia miltiorrhiza monomer IH764-3 induces hepatic stellate cell apoptosis via caspase-3 activation

机译:丹参单体IH764-3通过caspase-3激活诱导肝星状细胞凋亡

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CONCLUSION: Within the dose range used in present study, IH764-3 can inhibit HSC proliferation, as well as enhance HSC apoptosis. Furthermore, IH764-3 can significantly increase the caspase-3 protein expression. AIM: To investigate the effects of IH764-3 on HSC apoptosis, and the expression of caspase-3 protein in HSC apoptotic process. METHODS: HSCs were cultured in medium with different IH764-3 doses (10 μg·mL~(-1), 20 μg·mL(-1), 30 μg·mL~(-1), 40 μg· ML~(-1)) and without IH764-3, and HSC proliferation was quantitatively measured by ~3H-thymidine incorporation. The morphological changes of HSCs were observed with transmission electron microscope after exposure to the dose of 40 μg·mL~(-1) of IH764-3 for 48 hr.
机译:结论:在本研究使用的剂量范围内,IH764-3可以抑制HSC增殖,并增强HSC凋亡。此外,IH764-3可以显着增加caspase-3蛋白的表达。目的:探讨IH764-3对HSC凋亡的影响,以及caspase-3蛋白在HSC凋亡过程中的表达。方法:在不同剂量的IH764-3(10μg·mL〜(-1),20μg·mL(-1),30μg·mL〜(-1),40μg·ML〜(- 1))和没有IH764-3,并且通过〜3H-胸苷掺入来定量测量HSC增殖。暴露于40μg·mL〜(-1)IH764-3 48 h后,用透射电镜观察HSCs的形态变化。

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