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首页> 外文期刊>World Journal of Gastroenterology >Genetic evolution of structural region of hepatitis C virus in primary infection
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Genetic evolution of structural region of hepatitis C virus in primary infection

机译：初次感染中丙型肝炎病毒结构区的遗传进化

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CONCLUSION: Mutations of actively replicating virus arise stochastically with certain functional constaints. A complexity quasispecies exerted by a combination of either neutral evolution or selective forces shows clear differences in individuals, and associated with HCV persistence. AIM: To investigate the dynamics of hepatitis C virus (HCV) variability through putative envelope genes during primary infection and the mechanism of viral genetic evolution in infected hosts. METHODS: Serial serum samples prospectively collected for 12 to 34 months from a cohort of acutely HCV- infected individuals were obtained, and a 1-kb fragment spanning E1 and the 5′ half of E2, including Thirty-three cloned cDNAs representing each specimen were assessed by a method that combined a single-stranded conformational polymorphism (SSCP) and heteroduplex analysis (HDA) method to determine the number of clonotypes hypervariable region, was amplified by reverse transcriptase PCR and cloned.
机译：结论：具有一定功能限制的随机复制病毒突变是随机发生的。由中性进化或选择性力量共同作用所产生的复杂性准物种显示出个体之间明显的差异，并与HCV持续性有关。目的：通过原发性感染过程中的推定包膜基因研究丙型肝炎病毒（HCV）变异性的动态，以及被感染宿主中病毒遗传进化的机制。方法：从一组急性HCV感染者的前瞻性收集了12至34个月的连续血清样本，获得了一个跨越E1和E2的5'一半的1-kb片段，包括代表每个样本的三十三个克隆cDNA。通过结合单链构象多态性（SSCP）和异源双链分析（HDA）方法确定克隆型高变区数量的方法进行评估，通过逆转录酶PCR扩增并克隆。

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