• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bulletin of the National Research Centre >A Serine Protease from Germinated Wheat Seeds
【24h】

A Serine Protease from Germinated Wheat Seeds

机译：发芽的小麦种子中的丝氨酸蛋白酶

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A simple method for the purification of a serine endoprotease from wheat Triticum aestivum (cv. Giza 164) has been developed. It consists of ion-exchange and gel filtration chromatography. The molecular mass of the enzyme was 58 kDa by SDS/PAGE under reducing conditions and 57 kDa by gel filtration on a Sepharose 6B column. The enzyme had isoelectric point and pH optimum at 4.2 and 4.5, respectively. The substrate specificity of the enzyme was studied by the use of synthesized and natural substrates, azocasein, azoalbumin, hemoglobin, casein, gelatin and egg albumin. The enzyme appears to prefer azocasein with a K_m of 2 mg azocasein/ml. The enzyme had a temperature optimum at 50℃ with heat stability up to 40℃. While Co~(2+) and Mg~(2+) accelerated the enzyme activity by 54% and 56%, respectively, Ca~(2+) and Ni~(2+) had very little effect. The enzyme was strongly inhibited by PMSF (phenylmethylsulphonyl fluoride), but not by the other protease inhibitors, suggesting that the enzyme is a serine protease. From these results it can be concluded that the T. aestivum serine protease may be suitable for food processing.
机译：已经开发了一种从小麦小麦（cv。Giza 164）纯化丝氨酸内切蛋白酶的简单方法。它由离子交换色谱和凝胶过滤色谱组成。在还原条件下，通过SDS / PAGE，酶的分子量为58 kDa，在Sepharose 6B柱上，通过凝胶过滤，酶的分子量为57 kDa。该酶的等电点和pH最佳分别为4.2和4.5。通过使用合成的天然底物，偶氮酪蛋白，偶氮白蛋白，血红蛋白，酪蛋白，明胶和蛋清来研究酶的底物特异性。该酶似乎更喜欢偶氮酪蛋白，K_m为2 mg偶氮酪蛋白/ ml。该酶的最佳温度为50℃，热稳定性最高为40℃。 Co〜（2+）和Mg〜（2+）分别使酶活性提高了54％和56％，而Ca〜（2+）和Ni〜（2+）的作用很小。该酶被PMSF（苯基甲基磺酰氟）强烈抑制，但不受其他蛋白酶抑制剂的抑制，表明该酶是一种丝氨酸蛋白酶。从这些结果可以得出结论，普通小麦丝氨酸蛋白酶可能适用于食品加工。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号